畜牧兽医学报2025，Vol.56Issue(4)：1876-1886,11.DOI:10.11843/j.issn.0366-6964.2025.04.035
江西地区野猪菌群多样性分析及其携带产气荚膜梭菌的分离鉴定
Diversity Analysis of Wild Boar Flora in Jiangxi Region and the Isolation and Identification of Clostridium perfringens Carried by Wild Boar
摘要
Abstract
The aim of this study was to investigate the composition of the flora in the respiratory,intestinal and fecal tract of wild boars and the carriage situation of Clostridium perfringens(Cp).Respiratory,intestinal and fecal samples from twenty-seven wild boars were collected in Jiangxi region.Bacterial 16S rRNA gene sequencing technology was used to analyze the composi-tion and differences of the flora in different physiological parts of wild boars.And the Cp geno-types of wild boar sources and the carriage of toxin genes cpa,cpb,cpb2,etx,itx,cpe and netb were also analyzed.The Clostridium perfringens in the intestinal and fecal samples were isolated and purified,in additon,the whole-genome sequencing were conducted on the isolates.The re-sults of the flora analysis showed that the dominant phyla in all three groups were Firmicutes,Proteobacteria,Bacteroidetes and Actinobacteria,with the highest percentage of Firmicutes in the intestinal tract and feces and the highest percentage of Proteobacteria in the respiratory tract.Analysis of variance revealed highly significant differences in five genera in the three groups and significant differences in the remaining 10 genera;three Cp strains isolated from wild boar fecal samples were confirmed to be type A and were free of β2 toxin gene by PCR toxin genotyping and whole genome sequencing analysis;the carriage rate of Cp was 81.48％(22/27)in 27 samples,and the strains carried β2 toxin gene in these 22 positive samples were 9,representing 40.91％(9/22).No other toxin genes were detected.Combining the results of the toxin tests for each subtype,the Cp presented in the positive samples were all type A.In conclusion,based on the flora analysis,this study understood the microbial community composition of different physiolog-ical parts of wild boars and the carriage of Cp in wild boar,which provided theoretical clues for the prevention and detection of wild boar diseases.关键词
野猪/菌群分析/产气荚膜梭菌Key words
wild boar/microbial community analysis/Clostridium perfringens分类
畜牧业引用本文复制引用
谭娟娟,杨贝莹,武前悦,花慧颖,曹华斌,严珲,张锦华..江西地区野猪菌群多样性分析及其携带产气荚膜梭菌的分离鉴定[J].畜牧兽医学报,2025,56(4):1876-1886,11.基金项目
国家自然科学基金(32360880) （32360880）
江西省自然科学基金重点项目(20212ACB205005) （20212ACB205005）
