畜牧兽医学报2025，Vol.56Issue(4)：1887-1896,10.DOI:10.11843/j.issn.0366-6964.2025.04.036
毒害艾美耳球虫配子体抗原基因DNA疫苗的构建与免疫保护效果评价
Construction and Evaluation of the Immunoprotection of a DNA Vaccine Targeting Eimeria necatrix Gametocyte Antigen Gene
摘要
Abstract
The purpose of this experiment was to investigate the immunoprotection of a DNA vac-cine targeting Eimeria necatrix gametocyte antigen gene against coccidiosis.In this study,a DNA vaccine of gametocyte antigen gene,pVAX-Engam59,was constructed and combined with CpG ODN2006 adjuvant to immunize the chicks.The level of specific antibody in each group was detected by indirect ELISA.The mRNA transcription of cytokine(IL-4,IFN-γ,and TGF-β4)was analyzed by qRT-PCR.Finally,each chick was infected with 2.5×104 E.necatrix sporula-ted oocysts through the crop,and the clinical symptoms and bloody stools of each group were ob-served.The oocysts output,weight gains and lesion scores of each group were detected after 8 days post-infection.The results showed that the serum specific antibody and cytokine(IL-4,IFN-γ,and TGF-β4)levels of pVAX-Engam59 and pVAX-Engam59+CpG ODN2006 were sig-nificantly increased(P＜0.05),and the oocyst reduction ratios were 64.65％ and 64.48％,re-spectively.Compared to challenged and unchallenged control group,the number of bloody stools and the lesion scores in the pVAX-Engam59 and pVAX-Engam59+CpG ODN2006 groups were significantly lower(P＜0.05),and the relative weight gain rates were significantly higher(P＜0.05).The ACI values were 156.02 and 159.72,respectively.In summary,we successfully con-structed DNA vaccine targeting E.necatrix gametocyte antigenic gene,and both pVAX-Engam59+CpG ODN2006 and pVAX-Engam59 showed good immunoprotective effects,which were close to the anti-coccidial intermediate efficiency level,and could be candidates for a DNA vaccine against coccidiosis.关键词
毒害艾美耳球虫/配子体抗原基因/DNA疫苗/免疫保护Key words
Eimeria necatrix/gametocyte antigen gene/DNA vaccine/immunoprotection分类
畜牧业引用本文复制引用
冯永翠,刘丹丹,Naing Htet Aung,张馨尹,汪飞燕,王乐乐,张露,朱玉,许金俊,陶建平..毒害艾美耳球虫配子体抗原基因DNA疫苗的构建与免疫保护效果评价[J].畜牧兽医学报,2025,56(4):1887-1896,10.基金项目
国家自然科学基金(31972698 （）
31602039) （）
江苏省重点研发计划(现代农业,BE2021354) （现代农业,BE2021354）
江苏高校优势学科建设四期工程项目(2022-2025) （2022-2025）
高等学校学科创新引智计划资助(D18007) （D18007）
