畜牧兽医学报2025，Vol.56Issue(4)：1910-1918,9.DOI:10.11843/j.issn.0366-6964.2025.04.038
绵羊痒螨钙网蛋白对兔外周血单个核细胞Th1/Th2和Th17/Treg免疫平衡的影响
Effects of Recombinant Calreticulin of Psoroptes ovis on Th1/Th2 and Th17/Treg Immune Balance in Rabbit Peripheral Blood Mononuclear Cells
摘要
Abstract
The aim of this study was to investigate the effects of Psoroptes ovis calreticulin(PsoCRT)on Th1/Th2 and Th17/Treg immune balance in the peripheral blood mononuclear cells(PBMC)of healthy New Zealand rabbits.In this study,PsoCRT gene without signal pep-tide was amplified from mixed-stage P.ovis total RNA by reverse transcription PCR,and its re-combinant protein(rPsoCRT)was further expressed in prokaryotic expression and purified.Mo-reover,healthy rabbit PBMC were isolated and cultured,then added the different concentrations of rPsoCRT(5,10,20,40,and 80 μg·mL-1).Finally,the transcription levels of the primary cytokines(IFN-γ,IL-4,IL-17A,IL-10)and transcription factors(T-bet,GATA-3,RORc,Foxp3)of Th1,Th2,Th17 and Treg cells of rabbit PBMC were measured by qRT-PCR.Our re-sults showed that the full-length of PsoCRT was 1 254 bp,and Pso CRT belonged to the type cal-reticulin family.The purified rPsoCRT was of the expected molecular size of～64 ku.Rabbit PBMC treated with rPsoCRT upregulated the IFN-γ and T-bet mRNA expressions of Th1 cells,and decreased IL-4 mRNA expression while no different of GATA-3 mRNA expression in Th2 cells,which leaded to shifting T-cell differentiation towards Th1 bias with a drop in the IFN-γ/IL-4 and T-bet/GATA-3 ratios.Additionally,the mRNA expression of IL-17A in Th17 cells and Foxp3 in Treg cells were decreased,while the expressions of IL-10 and RORc remained un-changed,which resulted in shifting T-cell differentiation towards Treg bias with a drop in the IL-17A/IL-10 and RORc/Foxp3 ratios.These results indicated that rPsoCRT could cause imbalance in the transcription levels of Th1/Th2 and Th17/Treg primary cytokines and transcription factors in rabbit PBMC,resulting in host immunity shift to Th1 and Treg responses,respectively.关键词
绵羊痒螨/钙网蛋白/Th1/Th2/Th17/Treg/免疫平衡Key words
Psoroptes ovis/calreticulin/Th1/Th2/Th17/Treg/immune balance分类
农业科学引用本文复制引用
李艳娥,梁友萍,樊洁,吴芳燕,尧香悦,李毛却乎,次仁仓决,郝桂英,古小彬..绵羊痒螨钙网蛋白对兔外周血单个核细胞Th1/Th2和Th17/Treg免疫平衡的影响[J].畜牧兽医学报,2025,56(4):1910-1918,9.基金项目
四川省科技计划重点研发项目(2019YFN0155) （2019YFN0155）
四川农业大学学科双支计划(2221993268) （2221993268）
