畜牧兽医学报2025，Vol.56Issue(4)：1947-1957,11.DOI:10.11843/j.issn.0366-6964.2025.04.041
非细胞毒性浓度AFB1暴露下H1N1型猪流感病毒感染3D4/21细胞的蛋白质组分析
Proteomic Analysis of 3D4/21 Cells Infected with H1N1 Swine Influenza Virus under Non-cytotoxic Concentration of AFB1 Exposure
摘要
Abstract
Aflatoxin B1(AFB1)contamination is wide spread worldwide and seriously endangers the health of livestock and poultry.At present,more than 34 countries and regions in the world have established limit standards for the content of AFB1 in livestock and poultry feed.However,even exposure to AFB1 below the limit standard may still have the risk to promote infections of certain pathogens.Therefore,in this study,3D4/21 cells were used as a model.Firstly,the non-toxic concentrations of AFB1 were screened,and then the relative NP protein expression and ti-ters of swine influenza virus(SIV)were detected by Western blots and TCID50,respectively,thereby determining the effect of non-toxic concentrations of AFB1 exposures on SIV replication.Next,TMT technology under the proteomics platform was used to detect the differentially ex-pressed proteins and enrichment pathways between SIV infection and SIV+AFB1 groups,in or-der to explore the possible mechanism of AFB1 promoting SIV replication.The results showed that 0.01 μg·mL-1 AFB1 had no cytotoxicities on 3D4/21 cells and significantly promoted the replication of SIV in 3D4/21 cells;TMT results showed that 242 proteins were differentially up-regulated while 327 proteins were differentially down-regulated in the SIV+AFB1 group com-pared to SIV infection group.Further pathway enrichment analysis screened 137 pathways that affect the difference of AFB1-induced SIV replication.The top five enrichment pathways were proteasome pathway,necroptosis pathway,multi-species apoptosis pathway,legionella disease pathway,and drug metabolism-other enzyme pathway,respectively.Among them,the apopto-sis-related pathway may be the key pathway regulating AFB1 promoting SIV replication,and the subsequent DAPI staining test confirmed that.The research results will lay a foundation for early prevention and control of SIV infection aggravated by AFB1 pollution,and provide a reference for exploring the causes of the increase of other infectious diseases.关键词
黄曲霉毒素B1/H1N1/猪流感病毒/差异表达蛋白/细胞凋亡Key words
aflatoxin B1/H1N1/swine influenza virus/differentially expressed proteins/apoptosis分类
农业科技引用本文复制引用
庞思瑶,张金龙,孙雨航..非细胞毒性浓度AFB1暴露下H1N1型猪流感病毒感染3D4/21细胞的蛋白质组分析[J].畜牧兽医学报,2025,56(4):1947-1957,11.基金项目
辽宁省科技厅博士科研启动项目(2024BS094) （2024BS094）
辽宁省教育厅基本科研项目青年项目(Z20240295) （Z20240295）
国家自然科学基金青年项目(32002347) （32002347）
中国博士后基金面上项目(2023M741095 （）
2021M692231) （）
