畜牧兽医学报2025，Vol.56Issue(4)：1958-1968,11.DOI:10.11843/j.issn.0366-6964.2025.04.042
大车前苷抑制猪肺炎支原体诱导的炎性反应的作用初探
Preliminary Study on the Inhibitory Effect of Plantamajoside on Inflammatory Response Induced by Mesomycoplasma hyopneumoniae
摘要
Abstract
Mycoplasma hyopneumoniae(Mhp)colonizes to respiratory epithelial cells,causing the inflammatory injury of trachea,bronchus and lungs in pigs,which reduced the feed conver-sion rate and daily weight gain of pigs,resulting in serious economic losses to the swine industry.plantamajoside(PMS),as a commonly used anti-inflammatory traditional Chinese medicine,its role in inhibiting the inflammatory response caused by Mhp infection is unknown.The aim of this study was to investigate the effect and related mechanisms of PMS on inflammatory response in-duced by Mhp in vitro and in vivo.Mhp infected PK-15 cells and mice were used as infection models,respectively.First,PK-15 cells were treated with different concentrations of PMS to de-termine the maximum concentration of PMS that had no effect on cell morphology and viability.This highest concentration of PMS was then applied to PK-15 cells which infected with Mhp.The mRNA expression of tumor necrosisfactor-alpha(TNF-α),interleukin-6(IL-6)and other inflam-matory factors in PK-15 cells was detected by RT-qPCR.The results showed that the mRNA levels of IL-6,TNF-α,NF-κB and IκBα were significantly increased in PK-15 cells which infected with Mhp compared with the control group,while PMS could reduce the effect.Western blot and immunofluorescence were used to further analysis and the results showed that PMS could inhibi-ted the phosphorylation of NF-κB P65 and IKBα,and inhibited the nuclear translocation of NF-κB P65,which induced by Mhp.Related studies on mouse models have found that PMS can improve the lung lesions,reduce the inflammatory cells of lung which induced by Mhp.At the same time,PMS can significantly reduce the content of TNF-α in serum.These results suggest that PMS may ameliorate Mhp-induced inflammatory response through NF-κB pathway,which provides im-portant theoretical basis for novel prevention and treatment of Mhp infection.关键词
大车前苷(PMS)/猪肺炎支原体(Mhp)/肺炎/核因子-κBKey words
plantamajoside(PMS)/Mesomycoplasma hyopneumoniae(Mhp)/pneumonia/NF-κB分类
农业科技引用本文复制引用
王吉英,于岩飞,向书涵,李志强,李炻漾,张磊,谢青云,熊祺琰,邵国青,冯志新..大车前苷抑制猪肺炎支原体诱导的炎性反应的作用初探[J].畜牧兽医学报,2025,56(4):1958-1968,11.基金项目
湖南省自然科学基金项目(2022JJ50321) （2022JJ50321）
湖南省教育厅科学研究项目(22B1055) （22B1055）
江苏省科协青年科技人才托举工程(JSTJ-2023-XH031) （JSTJ-2023-XH031）
江苏省农业自主创新资金[CX(22)1011] （22）
怀化市科学技术局项目(2020R2206) （2020R2206）
