畜牧兽医学报2025，Vol.56Issue(4)：1969-1980,12.DOI:10.11843/j.issn.0366-6964.2025.04.043
呼宁散对鸡肺源大肠杆菌抑菌活性及体外抗炎、抗氧化作用研究
Study on the Activity of Huning Powder against Chicken Lung-derived E.coli and the in vitro Effects of Anti-inflammation and Anti-oxidation
摘要
Abstract
This study aimed to investigate the bacteriostatic activity of Huning Powder against E.coli of chicken lung origin,as well as the anti-inflammatory and anti-oxidation effects on lipopo-lysaccharide(LPS)-induced damage model of chicken embryonic fibroblasts(DF-1).Huning Powder was prepared with 10 Chinese herbs including Morus alba L,Scutellaria baicalensis Georgi,and Taraxacum.The antibacterial activity of Huning Powder against chicken lung-de-rived E.coli was determined by Oxford Cup method,the minimal inhibitory concentration(MIC)and bactericidal concentration(MBC)of Huning Powder was determined by TTC meth-od,and the bacterial growth curve was drawn.The contents of alkaline phosphatase(AKP),nu-cleic acid and soluble protein in bacterial culture medium were detected.The LPS-induced DF-1 cell damage model was established and drug was administered after the toxicity of LPS and Hu-ning Powder to DF-1 cells was detected by CCK-8 method.The levels of the inflammatory factors IL-1β,IL-6 and IL-8 were determined by ELISA.The expression of key proteins of Toll-like re-ceptor 4(TLR4)and nuclear factor kappa B p65(NF-κB p65)in the TLR4/NF-κB signaling pathway were determined by Western blot.The intracellular levels of reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD),and reduced glutathione(GSH)were determined by kits.The expression of key proteins of nuclear factor E2-related fac-tor 2(Nrf2)and Kelch-like ECH-associated protein 1(Keap1)in the Keap1/Nrf2 signaling path-way were determined by Western blot.Results were as follows:Chicken lung-derived E.coli was moderately susceptible to Huning Powder,and the MIC was 250 mg·mL-1 and MBC was 500 mg·mL-1.The growth of E.coli of chicken lung origin was inhibited and AKP,soluble protein and nucleic acid content in bacterial cultures were increased by Huning Powder.In cellu-lar assays,the levels of inflammatory factors IL-1β,IL-6,and IL-8 in the supernatant of LPS-in-duced DF-1 cells were reduced by Huning Powder.The overexpressions of TLR4 and NF-κB p65 proteins were inhibited by Huning Powder.The levels of ROS and MDA were reduced,SOD ac-tivity and GSH content were increased by Huning Powder.The expression of Nrf2,NQO1 and HO-1 proteins were increased and the overexpression of Keap1 protein was inhibited by Huning Powder.The traditional Chinese medicine compound Huning Powder had good bacteriostatic ac-tivity against chicken lung-derived E.coli and could exert anti-inflammatory and antioxidant effects by regulating the expression of key proteins of the TLR4/NF-κB and Keap1/Nrf2 signa-ling pathways in LPS-induced DF-1 cells.关键词
中药复方呼宁散/鸡肺源大肠杆菌/抑菌活性/抗炎/抗氧化Key words
Traditional Chinese medicine compound Huning Powder/chicken lung-derived E.co-li/antibacterial activity/anti-inflammation/anti-oxidation分类
农业科技引用本文复制引用
邱谦,桑锐,王巍,刘馨蔓,于明弘,刘晓童,于天,张雪梅..呼宁散对鸡肺源大肠杆菌抑菌活性及体外抗炎、抗氧化作用研究[J].畜牧兽医学报,2025,56(4):1969-1980,12.基金项目
吉林省自然科学基金(自由探索重点项目,YDZJ202401430ZYTS) （自由探索重点项目,YDZJ202401430ZYTS）
国家自然科学基金(32360900:32060817) （32360900:32060817）
