畜牧兽医学报2025，Vol.56Issue(4)：1981-1988,8.DOI:10.11843/j.issn.0366-6964.2025.04.044
公牛精子耐冻性相关基因多态性位点的挖掘与功能验证
Mining and Functional Verification of Gene Polymorphisms Loci Related to Bull Sperm Freezability
摘要
Abstract
The study aimed to identify key genes and molecular markers that affect the freezability of bull sperm and apply them to molecular breeding of dairy cattle.In this study,11 candidate genes related to bull sperm freezability were identified based on the previous omics study,such as ATP5F1A,DNASE2,and LPO.Primers were designed for the exons of these genes to detect genetic variation sites via PCR pooled sequencing.Subsequently,the Time-of-Flight mass spec-trometry was used to genotype important loci in a resource population of 130 healthy bulls and as-sociation analysis was performed.Among them,there were 55 bulls in the high sperm freezability group,with an average fresh sperm motility of 0.68 and an average post-freeze motility of 0.42,and 75 bulls in the low sperm freezability group,with an average fresh sperm motility of 0.65 and an average post-freeze motility of 0.32.Finally,14 significant SNPs were identified,among which the rs110909003 SNP(C/T)located in the exon of ATP5F1A was significantly associated with the bull sperm freezability(P=0.009).Western blot analysis was performed to detect the expression of ATP5F1A in sperm with different genotypes at the rs110909003 locus.The results showed a significant increase in ATP5F1A protein expression in sperm from bulls with the CC genotype compared to those with the TT genotype.This study identified ATP5F1A significantly associated with bull sperm freezability,as well as an important molecular marker,the rs1 10909003 SNP,located within this gene.This SNP may influence sperm freezability by affect-ing the mitochondrial oxidative phosphorylation process.These findings provide valuable infor-mation for the molecular breeding of frozen semen quality traits in Holstein bulls.关键词
荷斯坦牛/精子/耐冻性/功能基因/分子标记Key words
Holstein cattle/sperm/freezability/functional genes/molecular markers分类
农业科技引用本文复制引用
李晓彤,王鹏宇,方颖妍,于鸿希,张毅,王雅春,张元沛,李彦芹,姜力..公牛精子耐冻性相关基因多态性位点的挖掘与功能验证[J].畜牧兽医学报,2025,56(4):1981-1988,8.基金项目
国家重点研发计划项目(2022YFD1302200) （2022YFD1302200）
国家现代农业产业技术体系(CARS-36) （CARS-36）
