畜牧兽医学报2025，Vol.56Issue(4)：1989-1994,6.DOI:10.11843/j.issn.0366-6964.2025.04.045
水獭源犬圆环病毒的全基因组序列分析
Whole Genome Sequence Analysis of Canine Circovirus in an Otter(Lutra lutra)
摘要
Abstract
Canine circovirus(CanineCV)is a virus associated with canine gastrointestinal disea-ses.Since it was first identified from dogs in 2012 in the United States.Researchers have subse-quently detected the presence of CanineCV in various wildlife species,including wolves(Canis lupus),badgers(Meles meles),red foxes(Vulpes vulpes),arctic foxes(Vulpes lagopus)and jackals(Lupulella mesomelas).In 2016,CanineCV was first reported in dogs in China.Howev-er,a systematic investigation of CanineCV infection in wildlife in China remains lacking.We ac-quired tissue specimens from an ill and deceased otter(Lutra lutra),and successfully identified the presence of CanineCV through the application of Polymerase Chain Reaction(PCR).We de-signed of CanineCV genome-wide primers for whole genome amplification,sequenced and ana-lyzed the direction of genetic evolution.Result were as follows:Through PCR technology,we tested the otter tissue samples for CanineCV,and the results were positive.By whole-genome amplification,sequencing,and assembly,we successfully obtained a CanineCV whole-genome se-quence with a length of 2 063 nt.Nucleotide homology analysis revealed that this strain had a gene sequence homology of 84.6％-98.2％ with all other strains in GenBank.After constructing a genetic evolution tree,we found that this strain belonged to CanineCV-3.This is the first iden-tification of CanineCV infection in an otter,and we have successfully obtained the first whole-ge-nome sequence and genetic evolution information of CanineCV derived from wildlife in China.The above results illustrate the potential hazards of CanineCV infecting otters and their possibili-ty of infecting more other animals,thus suggesting that we should strengthen the monitoring of CanineCV infection in different animals.关键词
犬圆环病毒/水獭/流行病学/遗传进化分析Key words
canine circovirus/Lutra lutra/epidemiology/genetic evolutionary analysis分类
农业科技引用本文复制引用
匡尹之,徐凤培,周沛..水獭源犬圆环病毒的全基因组序列分析[J].畜牧兽医学报,2025,56(4):1989-1994,6.基金项目
广州市科技局基础与应用基础专题(SL2022A04J00674) （SL2022A04J00674）
广东省自然科学基金(2023A1515012171) （2023A1515012171）
