生物加工过程2025，Vol.23Issue(2)：137-146,10.DOI:10.3969/j.issn.1672-3678.2025.02.002
酿酒酵母中尿嘧啶基因表达的转录组分析及促生长差异基因挖掘
Transcriptome analysis for the expression of uracil in Saccharomyces cerevisiae and mining of growth-related differential genes
摘要
Abstract
Cell density is one of the most important factors affecting yield in microbial fermentation.During the fermentation of Saccharomyces cerevisiae,some additives to the yeast peptone dextrose(YPD)medium,such as uracil,could promote the strain growth.In addition,the S.cerevisiae strain ZL2,capable of expressing the uracil synthesis gene,also displayed a growth advantage over the control strain ZL1.In this study,transcriptome sequencing was performed on both ZL2 and ZL1 strains to investigate the reasons for growth advantages and figure out potential growth-promoting genes.According to the transcriptome analysis,there were 256 significantly up-regulated genes and 634 significantly down-regulated genes.Further analysis of these significantly differential genes was conducted using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)approaches,to explore their functions and related metabolic pathways.Four up-regulated genes and four down-regulated genes,which were identified to be responsible for cell growth,were subjected to further validation in strain ZL1.As a result,all eight genes could significantly promote cell growth and increase the production of ergosterol and β-amyrin in the engineered strain.Our studies will provide scientific support to improve cell biomass in biosynthesis and enhance the ability of microbial cells to synthesize natural products.关键词
细胞生长/转录组测序/酿酒酵母/差异基因/尿嘧啶Key words
cell growth/transcriptomic sequencing/Saccharomyces cerevisiae/differential genes/uracil分类
生物工程引用本文复制引用
李金玲,臧国伟,王颖..酿酒酵母中尿嘧啶基因表达的转录组分析及促生长差异基因挖掘[J].生物加工过程,2025,23(2):137-146,10.基金项目
国家自然科学基金(22078020) （22078020）
国家重点研发计划(2019YFA0905700) （2019YFA0905700）
