生物加工过程2025，Vol.23Issue(2)：199-206,8.DOI:10.3969/j.issn.1672-3678.2025.02.010
沙苑子提取物对糖尿病所导致睾丸损伤的治疗作用
Therapeutic effect of Semen Astragall Complanati extract on diabetes-induced testicular injury
摘要
Abstract
Semen Astragall Complanati(SAC)extract was studied with regards to its protective effect and mechanism on diabetes-induced testicular injury.The type 2 diabetic mouse model was established by feeding with high-fat diet and intraperitoneal injection of streptozotocin(STZ).Male ICR mice were randomly divided into normal group,model group and three SAC groups(100,200 and 400 mg/kg SAC),respectively.The mice in SAC groups were administrated intragastrically for 8 weeks,while those in the normal and model groups were dosed with normal saline.At the end of treatment,the blood glucose levels,testis index,sperm count and morphology were detected,and the biochemical indicators including malondialdehyde(MDA),H2O2,catalase(CAT)were measured by Biochemical kit assay.The testis tissue slices were subsequently analyzed using HE staining experiments,and the contents of 8-OHdG and 4-HNE were detected by immunohistochemistry.To further explore the mechanism of action,Nox4 mRNA and protein level were detected by RT-qPCR and Western blotting assay,respectively.Compared with the model group,SAC treatment helped the recovery from diabetic testicular injury based on histological pathology,with significant improvement for testicular index and sperm quality.Moreover,SAC could not only significantly attenuate the MDA and H2O2 contents but also enhance CAT activities in the testis tissue.Based on the immunohistochemical studies using 8-OHdG and 4-HNE assay,the SAC extract could inhibit oxidative stress damage caused by diabetes to a certain extent.In addition,SAC could inhibit the abnormally elevated protein expression and the Nox4 level in testis tissue.Overall,our research suggests that SAC extract is a potential therapeutic agent in the treatment of diabetes-induced testicular injury through the modulation of Nox4 level and oxidative stress.关键词
沙苑子提取物/糖尿病/Nox4/睾丸损伤/氧化应激Key words
Semen Astragall Complanati(SAC)extract/diabetes/Nox4/testicular injury/oxidative stress分类
药学引用本文复制引用
李佳琪,莫廷美,王小轩,林娜,韦景薰,闫炳荣,傅杨恺,黄鹭铭,马博..沙苑子提取物对糖尿病所导致睾丸损伤的治疗作用[J].生物加工过程,2025,23(2):199-206,8.基金项目
江苏省高等学校基础科学(自然科学)面上项目(21KJB350020) （自然科学）
江苏省先进生物制造创新中心重点项目(XTC2203) （XTC2203）
南京市科技局生命健康科技专项(202110015) （202110015）
江苏省大学生创新创业训练计划重点项目(202110291056Z) （202110291056Z）
