生物加工过程2025，Vol.23Issue(2)：207-211,5.DOI:10.3969/j.issn.1672-3678.2025.02.011
转录因子YAP1在X线诱导的HaCaT细胞损伤中的作用和作用机制
Study on the role and mechanism of transcription factor YAP1 in X-ray-induced damage of HaCaT cells
摘要
Abstract
Radiation-induced skin injury is a common complication of radiotherapy.In this study,using HaCaT cells,a cell model of radiation-induced skin injury was developed through in vitro X-ray irradiation.The inhibitory effects of X-rays on the viability and proliferation capacity of HaCaT cells were validated through the MTT colorimetric assay,EDU proliferation assay,and colony formation assay.DCFH-DA and DHE probes were used to assess the effects of X-ray on intracellular reactive oxygen species(ROS)levels.The mechanism of X-ray-induced inhibition of cell proliferation by RT-qPCR,Western-blotting,and immunofluorescence assay.The results showed that X-ray inhibited the viability and proliferation capacity of HaCaT cells,induced an increase in intracellular ROS levels,and downregulated the expression of CyclinD1 and CyclinE1.Moreover,X-ray inhibited the expression and nuclear translocation of the transcription factor YAP1,which may be an important reason for the reduced cell proliferation capacity.This study revealed the molecular mechanism of X-ray-induced damage of HaCaT cells and identified a molecular target for the prevention and treatment of radiation-induced skin injury.关键词
放射损伤/HaCaT细胞/YAP1/细胞增殖/氧化应激Key words
radiation injury/HaCaT cells/YAP1/cell proliferation/oxidative stress分类
药学引用本文复制引用
黄腾,于大海,王万霞,鹿红..转录因子YAP1在X线诱导的HaCaT细胞损伤中的作用和作用机制[J].生物加工过程,2025,23(2):207-211,5.基金项目
国家自然科学基金青年项目(82405508) （82405508）
江苏省中医药管理局科技项目(YB2020009、YB201815) （YB2020009、YB201815）
