畜牧兽医学报2025，Vol.56Issue(4)：1494-1507,14.DOI:10.11843/j.issn.0366-6964.2025.04.003
3D培养在家畜卵母细胞及胚胎培养的研究现状与应用前景
The Research Status and Application Prospect of 3D Culture in Livestock Oocytes and Embryos Culture
摘要
Abstract
Three-dimensional(3D)cell culture technology can accurately simulate the actual mi-croenvironment of cells and reproduce the interactions between cells,so that the behavior charac-teristics of cells are more similar to the living state in vivo.Therefore,3D cell culture technology has been widely used in the research of oocyte and embryo development in domestic animals,which has greatly improved the quality of oocyte and embryo development in vitro,and thus pro-moted the consistency and reliability of research results.This paper discusses the development of 3D in vitro culture from three aspects:materials,substrates and methods,and summarizes new classification methods based on the biological characteristics of cells,culture conditions,scaffold materials used,interactions between cells and the design of culture systems,namely:cell aggre-gation system,construction of structural scaffolds,construction of bionic scaffolds,and multi-disciplinary intersections(extension).In the future,through the improvement and optimization of 3D model cell culture technology,it will provide important technical support and new possibili-ties for the research of in vitro culture of different domestic oocytes and embryonic development.关键词
三维培养系统/卵母细胞/体外胚胎Key words
three-dimensional culture system/oocytes/in vitro embryos分类
农业科技引用本文复制引用
闫瑞,贾朝阳,马静,杨娟,刘新峰,陈强..3D培养在家畜卵母细胞及胚胎培养的研究现状与应用前景[J].畜牧兽医学报,2025,56(4):1494-1507,14.基金项目
宁夏回族自治区重点研发计划项目(引才专项)(2024BEH04111) （引才专项）
国家自然科学基金项目(32160791) （32160791）
宁夏自然科学基金重点项目(2024AAC02019) （2024AAC02019）
