现代医药卫生2025，Vol.41Issue(4)：828-835,8.DOI:10.3969/j.issn.1009-5519.2025.04.003
基于转录组测序分析芹菜素抑制巨噬细胞焦亡的潜在作用机制
The potential mechanism of apigenin inhibits macrophage pyroptosis based on transcriptome sequencing
摘要
Abstract
Objective To explore the effect and mechanism of apigenin on macrophage pyroptosis.Methods THP-1 cells were cultured in vitro and induced to differentiate into macrophages by phorbol ester,and the macrophages were induced to pyroptosis by lipopolysaccharide(LPS)and nigericin.The cell morpholo-gy was observed by optical microscope.Western blot was used to detect the Gasdermin D-N(GSDMD-N).The mRNA expressions of NLRP3,IL-1β and TNF-α were detected by real-time fluorescent quantitative polymer-ase chain reaction(qRT-PCR)to investigate the effect of apigenin on macrophage pyroptosis.Transcriptome sequencing was further used to screen differentially expressed genes and signaling pathways.Finally,qRT-PCR was used to verify the expression of 5.8S rRNA,18S rRNA,28S rRNA,Pre-45S rRNA and POP1,NAT10,RCL1 and GAR1 related genes,and to explore the role of ribosome biosynthesis signaling pathway in apigenin inhibiting macrophage pyroptosis.Results Apigenin pretreatment inhibited the formation of bubble-like protrusions during macrophage pyroptosis,and reduced the expression of GSDMD-N protein and the mR-NA expression of NLRP3,IL-1β,and TNF-α.Gene ontology(GO)enrichment analysis showed that apigenin was mainly involved in nucleic acid metabolic process,RNA metabolic process and transcription regulator ac-tivity.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that apigenin mainly participates in ribosome biogenesis,NOD-like receptor signaling pathway and Toll-like receptor signaling pathway related to pyroptosis.qRT-PCR showed that apigenin pretreatment significantly reduced the expres-sion of 5.8S rRNA,18S rRNA,28S rRNA,Pre-45S rRNA,RCL1,GAR1,POP1 and NAT10.Conclusion Api-genin has an inhibitory effect on macrophage pyroptosis,and its mechanism may be related to the regulation of the ribosome biogenesis signaling pathway.关键词
巨噬细胞/细胞焦亡/芹菜素/转录组测序/核糖体生物合成信号通路Key words
Macrophage/Pyroptosis/Apigenin/Transcriptome sequencing/Ribosome biogene-sis signaling pathway分类
医药卫生引用本文复制引用
白勒谢,吴帅凯,叶利经,潘玉芝,吴丹媚,赵国军..基于转录组测序分析芹菜素抑制巨噬细胞焦亡的潜在作用机制[J].现代医药卫生,2025,41(4):828-835,8.基金项目
国家自然科学基金项目(81870337) （81870337）
广东省自然科学基金面上项目(2024A1515012850). （2024A1515012850）
