应用化学2025，Vol.42Issue(4)：511-521,11.DOI:10.19894/j.issn.1000-0518.240394
基于硅量子点荧光法和比色法双模式检测绿原酸
Dual Mode Detection of Chlorogenic Acid Based on Silicon Quantum Dot via Fluorescence Spectroscopy and Colorimetric Method
摘要
Abstract
A silicon quantum dots(SiQDs)was prepared by refluxing 3-aminopropyltriethoxysilane(APTES),sodium ascorbate and sodium citrate with heating at 90℃.Based on the SiQDs,an efficient,sensitive and convenient method for the detection of chlorogenic acid(CGA)was constructed based on the dual-mode fluorescence and UV absorption via electrostatic adsorption between SiQDs and CGA.The SiQDs possessed good water solubility as well as excellent selectivity for CGA.The fluorescence quantum yield(QD)of SiQDs was as high as 0.58.Under the optimal working conditions,the linear range of fluorescence detection of SiQDs was 0.054～265 μmol/L,and the limit of detection was 0.018 μmol/L.The absorbance detection of SiQDs was 0.516～45 μmol/L,and the limit of detection was 0.172 μmol/L.In addition,the analytical detection of CGA in Lonicera japonica Thunb.was realized by using the spiked recovery method,which showed good recoveries(97.76%～105.5%).关键词
硅量子点/3-氨丙基三乙氧基硅烷/绿原酸/荧光探针Key words
Silicon quantum dots/3-Aminopropyltriethoxysilane/Chlorogenic acid/Fluorescent probe分类
化学引用本文复制引用
王松柏,李天宇,魏梦姝,郭炘明,李俊芬,周影,彭秀英,董川..基于硅量子点荧光法和比色法双模式检测绿原酸[J].应用化学,2025,42(4):511-521,11.基金项目
山西省回国留学人员科研项目(Nos.2021-011,2021-012)和山西省基础研究计划(Nos.202103021223351,20210302123447)资助 Supported by Shanxi Scholarship Council of China(Nos.2021-011,2021-012)and the Basic Research Project of Shanxi Provincial(Nos.202103021223351,20210302123447) （Nos.2021-011,2021-012）
