应用化学2025，Vol.42Issue(4)：522-532,11.DOI:10.19894/j.issn.1000-0518.240417
面向生物样品分析的小檗碱的电化学-荧光双模传感器
An Electrochemical-Fluorescence Dual-Mode Chemosensor for Berberine Detection in Biological Samples
摘要
Abstract
By taking advantage of the non-covalent interactions between 1,3,6-tri-sulfonate 8-aminonaphthalene(ANTS)and berberine,we have reported a novel electrochemical-fluorescent dual-mode chemosensor for berberine based on using ANTS as both the recognition element for berberine and a dual-mode probe for electrochemical and fluorescent responses.ANTS was immobilized onto the glass carbon(GC)electrode surface via single-walled carbon nanotubes(SWNTs)through π-π interactions to generate the ANTS/SWNTs/GC electrode.Upon being immersed in berberine solution,the ANTS/SWNTs-modified GC electrode shows current decreases in the oxidation peaks at both-0.003 V and+0.074 V(vs.Ag/AgCl)due to the interaction-driven adsorption of berberine on the electrode surface,which validates berberine detection using the sum of the ratios of current decreases in two oxidation peaks as the electrochemical signal readout.Upon addition of berberine into ANTS solution,ANTS emission shows a turn-off signal at 517 nm,which enables the berberine quantification using the decrease in ANTS emission at 517 nm as the fluorescent readout.Under the optimized conditions,the ANTS-based electrochemical sensor shows current decrease response to berberine in the dynamic range from 0.05 to 1000 μmol/L with a detection limit of 0.013 μmol/L,and the fluorescent sensor shows linear fluorescent response to berbeine within a concentration range of 1～100 μmol/L with a detection limit of 0.325 μmol/L.In the animal experiments,the dual-mode chemsensor has demonstrated a high selectivity against the endogenous species coexisting in rat plasma and liver homogenerates.Moreover,the electrochemical sensor is remarkably sensitive for berberine level quantification in rat plasma and liver homogenates,wherein the relative standard deviations(RSDs)for the plasma and liver homogenates measurements are 3.0%(n=3)and 4.0%(n=3),respectively.The fluorescent sensor has achieved satisfactory spike-and-recoveries within 99.8%～100.2%(n=3)for plasma and 95.5%～99.9%(n=3)for liver homogenates.The dual-mode chemosensor reported here is readily accessible,simple,robust,and cost-effective and offers a promising tool for rapid detection of berberine in biological samples with less technically demanding and simple operating procedures.关键词
1,3,6-三磺酸8-氨基萘/小檗碱/双模传感器/荧光/电化学传感器Key words
1,3,6-Tri-sulfonate 8-aminonaphthalene/Berberine/Dual-readout sensor/Fluorescence/Electrochemical sensor分类
化学化工引用本文复制引用
彭文琪,周锐,程旺兴,张自品..面向生物样品分析的小檗碱的电化学-荧光双模传感器[J].应用化学,2025,42(4):522-532,11.基金项目
安徽省自然科学基金面上项目(No.2008085MH293)、安徽省高校自然科研项目重点项目(No.2022AH050459)和安徽省2020年省级质量工程项目-分析化学大规模在线开放课程(MOOC)示范项目(No.2020MOOC265)资助 Supported by the Natural Science Foundation of Anhui Province of China(No.2008085MH293),the Key Projects of Natural Scientific Research Programs of Universities in Anhui Province(No.2022AH050459)and Anhui Province 2020 Provincial-Level Quality Engineering Project-Analytical Chemistry Massive Open Online Caurse(MOOC)Demonstration Project(No.2020MOOC265) （No.2008085MH293）
