畜牧兽医学报2025，Vol.56Issue(4)：1621-1631,11.DOI:10.11843/j.issn.0366-6964.2025.04.013
环介导等温扩增技术的发展及其在耐药基因检测中的应用
Development of Ring-mediated Isothermal Amplification Technology and Its Application to the Detection of Drug Resistance Genes
摘要
Abstract
This paper describes the principle,process and characteristics of loop-mediated isother-mal amplification(LAMP)technology and its application in the detection of drug resistance genes.LAMP technology has been widely used in the field of molecular biology due to its high ef-ficiency,specificity,sensitivity and simplicity.In addition to the basic LAMP detection methods,some novel LAMP technologies,such as IMS-LAMP,LAMP coupled with CRISPR/Cas system,LAMP coupled with Argonaute system,LAMP sensors,and LAMP coupled with microfluidic chip,are also introduced in this paper.In addition,for the detection of drug-resistant genes,LAMP technology shows a good application prospect in the detection of drug resistance genes,which provides an effective tool for rapid and accurate detection of drug resistance genes.关键词
环介导等温扩增/恒温扩增/快速扩增/检测方法/耐药基因检测Key words
loop-mediated isothermal amplification/constant temperature amplification/rapid am-plification/detection methods/drug resistance gene detection分类
农业科技引用本文复制引用
廖怡雯,叶景芬,武绍碧,陈世雄,杨婉,罗雪,杨琦..环介导等温扩增技术的发展及其在耐药基因检测中的应用[J].畜牧兽医学报,2025,56(4):1621-1631,11.基金项目
国家自然科学基金(32260884) （32260884）
