畜牧兽医学报2025，Vol.56Issue(4)：1689-1699,11.DOI:10.11843/j.issn.0366-6964.2025.04.018
民猪猪繁殖与呼吸综合征恢复力高低组血清代谢组学研究
Study on Serum Metabolomics of High and Low Resilience Group of Min Pigs with Porcine Reproductive and Respiratory Syndrome
摘要
Abstract
The aim of this study was to study the serum metabolomics of high and low resilience groups of porcine reprodective and respiratory syndrome(PRRS,blue ear disease)in Min pig,and to provide reference for the prevention and control of blue ear disease.In this study,from the perspective of metabolomics,15 Min pigs aged 28 days with similar body weights were selected,the same feeding environment,and the same exposure to porcine reproductive and respiratory syndrome virus(PRRSV).Among them,5 pigs were classified into the high-resilience group and 5 into the low-resilience group.Non-targeted metabolomic analysis of serum samples was per-formed using liquid chromatography-mass spectrometry(LC-MS).Differential metabolites in the serum of Min pigs with different resilience levels were screened through orthogonal partial least squares discriminant analysis(OPLS-DA)and cluster analysis,followed by pathway enrichment analysis.The results identified 39 differential metabolites between resilience groups(P＜0.05).Analysis revealed that sphingolipids,indigo carmine,arachidonic acid,and L-threonine were po-tential biomarkers.Further analysis indicated that pathways related to valine,leucine,isoleucine biosynthesis,and arachidonic acid metabolism might alleviate inflammatory responses by modula-ting cytokine levels and inflammatory mediators,thereby affecting resilience.In summary,this study identified sphingomyelin,indigo,arachidonic acid,and other metabolites associated with resili-ence to blue ear disease.Additionally,it provided a preliminary interpretation of the mechanisms underlying differences in resilience from a metabolic perspective.This research offers theoretical insights for both the prevention and control of blue ear disease and the breeding of Min pigs with high resilience.关键词
民猪/猪繁殖与呼吸综合征/恢复力/代谢组学Key words
Min pigs/PRRS/recovery ability/metabolomics分类
农业科技引用本文复制引用
孟祥旭,李佳,任德明,陈奎蓉,和艺云,王立贤,盛熙晖,王立刚..民猪猪繁殖与呼吸综合征恢复力高低组血清代谢组学研究[J].畜牧兽医学报,2025,56(4):1689-1699,11.基金项目
国家生猪产业技术体系(CARS-35) （CARS-35）
