畜牧兽医学报2025，Vol.56Issue(4)：1712-1721,10.DOI:10.11843/j.issn.0366-6964.2025.04.020
高温应激下湖羊卵巢颗粒细胞m6A甲基化修饰差异研究
Study on the Difference of m6A Methylation Modification in Ovarian Granulosa Cells of Hu Sheep under Heat Stress
摘要
Abstract
The aim of this study was to investigate the effect of heat stress on m6A methylation modification in Hu sheep ovarian granulosa cells,so as to lay the foundation for revealing the regula-tory mechanism of m6A methylation modification on the development and function of Hu sheep ovarian granulosa cells under heat stress.In this study,fresh ovaries of 2-year-old Hu ewes were collected,and granulosa cells in 3-5 mm follicles on the surface of ovaries were collected.They were randomly divided into two groups:control group(37 ℃)and heat stress group(42 ℃,2 h per day for 3 consecutive days).The m6A peak was identified by methylated RNA immunopre-cipitation sequencing(MeRIP-seq),and the gene expression data were obtained and analyzed.There were 135 significantly different m6A peaks in MeRIP-seq between the control group and the heat stress group,which were mapped to 130 differentially methylated genes,mainly en-riched in the PI3K-Akt signaling pathway,glutathione metabolism and Wnt signaling pathway.RNA-seq identified 359 significantly differentially expressed genes,which were mainly enriched in steroid synthesis,cholesterol biosynthesis and other signaling pathways.The combined analy-sis of MeRIP-seq and RNA-seq data showed that there were significant differences in m6 A modifi-cation and gene expression levels of five genes,DTX3L,MAGEF1,MAN1C1,CCDC77 and RAD51,which were mainly enriched in the Notch signaling pathway.This study results shows that heat stress can change the mRNA m6A modification level of Hu sheep ovarian granulosa cells,which may eventually affect cell proliferation,apoptosis,estrogen synthesis and other re-lated pathways,providing a theoretical basis for further exploring the specific mechanism of m6A modification under heat stress.关键词
高温应激/卵巢颗粒细胞/湖羊/m6A甲基化修饰/MeRIP-seqKey words
heat stress/ovarian granulosa cells/Hu sheep/m6A methylation modification/MeRIP-seq分类
农业科技引用本文复制引用
李笑微,田微,刘媛,李惠侠..高温应激下湖羊卵巢颗粒细胞m6A甲基化修饰差异研究[J].畜牧兽医学报,2025,56(4):1712-1721,10.基金项目
国家自然科学基金(32472963) （32472963）
