畜牧兽医学报2025，Vol.56Issue(4)：1722-1730,9.DOI:10.11843/j.issn.0366-6964.2025.04.021
miR-215-5p在雷州黑鸭组织中的表达及其对卵泡颗粒细胞增殖和凋亡的影响
Expression of miR-215-5p in Leizhou Black Duck Tissues and Its Effect on Follicular Granulosa Cells Proliferation and Apoptosis
摘要
Abstract
The study aimed to investigate the expression pattern of miR-215-5p in tissues of Leizhou black ducks and verify the regulatory effect of miR-215-5p on the proliferation and apop-tosis of follicular granulosa cells.Three 43-week-old egg-laying and three 51-week-old non-laying Leizhou black ducks with similar body weight and health conditions were selected.The expres-sion levels of miR-215-5p in different tissues were detected using RT-qPCR.By constructing mimics and interference fragment vectors and transfecting them into follicular granulosa cells,cell proliferation and apoptosis were detected using CCK-8 and flow cytometry techniques,with each group containing 3 replicates.The results showed that the expression of miR-215-5p in egg-laying Leizhou black ducks was generally higher than that in non-laying ducks,with relatively high expression in the ovary,infundibulum,and breast leg muscles,and relatively low expres-sion in the funnel part,uterus,and heart.CCK-8 and EdU assays showed that miR-215-5p inhib-ited the proliferation of follicular granulosa cells;flow cytometry detected that miR-215-5p pro-moted the apoptosis of follicular granulosa cells;RT-qPCR detection of proliferation and apopto-sis marker genes expression showed that after transfection with miR-215-5p mimics,the expres-sion levels of CCND1 and CDK2 were both significantly decreased(P＜0.01),and Caspase-3 was significantly increased(P＜0.01);while after transfection with miR-215-5p interference fragments,CCND1 was significantly increased(P＜0.05),CDK2 was significantly increased(P＜0.01),Caspase-3 was significantly decreased(P＜0.01),and TP63 was significantly de-creased(P＜0.05).miR-215-5p in Leizhou black duck has the ability to restrain the proliferation of follicular granulosa cells and promote their apoptosis.关键词
雷州黑鸭/miR-215-5p/miRNA/颗粒细胞/增殖凋亡Key words
Leizhou black duck/miR-215-5p/miRNA/granulosa cells/proliferation and apoptosis分类
农业科技引用本文复制引用
叶润根,刘渊博,路丽丽,Collins Amponsah Asiamah,苏瑛..miR-215-5p在雷州黑鸭组织中的表达及其对卵泡颗粒细胞增殖和凋亡的影响[J].畜牧兽医学报,2025,56(4):1722-1730,9.基金项目
广东省科技计划项目(2017A020208066) （2017A020208066）
广东省教育厅基础研究重大项目及应用研究重大项目(自然科学类)(2017KZDXM043) （自然科学类）
