中国农业科技导报2025，Vol.27Issue(5)：182-192,11.DOI:10.13304/j.nykjdb.2023.0863
分心木水提物的抗氧化性分析及组分鉴定
Oxidation Resistance Analysis and Component Identification of Distracted Wood Water Extracts
苗春霖 1许欢欢 1贾紫毅 1何爱民 2吉洋洋 2牟德华 3高山1
作者信息
- 1. 河北科技大学食品与生物学院,石家庄 050091
- 2. 河北绿岭康维食品有限公司,河北 邢台 054300||河北省(邢台)核桃产业技术研究院,河北 邢台 054300
- 3. 河北省(邢台)核桃产业技术研究院,河北 邢台 054300
- 折叠
摘要
Abstract
In order to study the optimal extraction process and oxidation resistance of the aqueous extract of diaphragma juglandis fructus(DJF),the liquid-material ratio,ultrasonic time and ultrasonic temperature were selected as the influencing factors,and the free radical clearance rate of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)was used as the response value.The response surface method was used to optimize the process conditions of ultrasonic-assisted extraction of DJF water extracts,and the component analysis and oxidation resistance of the water extracts from distracted wood were studied.The results showed that the optimal process for determining the aqueous extract of DJF by the Box-Benhnken model was as follows:liquid-material ratio of 92 mL:1 g,ultrasonic temperature of 58℃,ultrasonic time of 19 min,and the obtained DPPH free radical clearance rate was 68.95％.High performance liquid chromatography(HPLC)showed that the content of polyphenols in the DJF water extracts was 0.28 mg·mL-1,and the contents of polysaccharides were 0.33 mg·mL-1,and the main active components were quercetin 245 µg·mg-1,gallic acid 18 µg·mg-1 and galactose 98 µg·mg-1.The results of DJF water extract antioxidant capacity test showed that the free radical clearance rate of DPPH and free radical clearance rate of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS)of DJF water extract were generally lower than those of vitamin C(Vc),and the total reducing power was greater than that of Vc,indicating that it had better antioxidant capacity and it was a potential antioxidant food quality raw material.Above results provided data support for the extraction of DJF active ingredients,and provided a theoretical basis for further exploration of DJF functional components.关键词
核桃分心木/水提物/响应面/抗氧化/液相色谱Key words
diaphragma juglandis fructus/water extracts/response surface/antioxidant/liquid chromat-ography分类
农业科学引用本文复制引用
苗春霖,许欢欢,贾紫毅,何爱民,吉洋洋,牟德华,高山..分心木水提物的抗氧化性分析及组分鉴定[J].中国农业科技导报,2025,27(5):182-192,11.
