中国农业科技导报2025，Vol.27Issue(5)：222-230,9.DOI:10.13304/j.nykjdb.2025.0159
饲料中添加壳寡糖对斑马鱼肝肠健康和抗病能力的影响
Effects of Chitosan Oligosaccharides in Dietary on Hepatointestinal Health and Disease Resistance of Zebrafish
摘要
Abstract
To investigate the effects of dietary supplementation with chitosan oligosaccharide on the hepatointestinal health and disease resistance of zebrafish,a 28-day feeding trial was conducted,which designed 4 treatment groups with chitosan oligosaccharide supplementation at mass fractions of 0(control),0.05%,0.10%and 0.20%.The results showed that feeding chitosan oligosaccharide had no significant effect on the growth of zebrafish(P>0.05).However,it enhanced the antioxidant capacity of zebrafish,significantly reduced the content of malondialdehyde in the liver and intestine(P<0.05),increased the expression of the anti-inflammatory factor gene(transforming growth factor tgf-β)in the intestine,and significantly decreased the expression of pro-inflammatory factor genes(nuclear transcription factor nf-κb,tumor necrosis factor tnf-α and interleukin il-1β).In addition,dietary chitosan oligosaccharide increased the expression of genes related to hepcidin,lysozyme(LYS),zonula occludens-1(zo-1),and occladin(occludin)in the intestine of zebrafish,as well as the survival rate after pathogenic bacterial infection.The results indicated that the addition of 0.10%chitosan oligosaccharide to the feed enhances the non-specific immunity of zebrafish,improves liver and intestinal health,and the ability to resist Aeromonas veronii infection,providing a reference for the safe and efficient promotion and utilization of chitosan oligosaccharide in aquaculture.关键词
壳寡糖/斑马鱼/肝肠健康/非特异性免疫/抗病力Key words
chitosan oligosaccharides/zebrafish/liver and intestinal health/non-specific immunity/disease resistance分类
水产学引用本文复制引用
符广才,李道君,杨宏伟,李生辉,张青双,张震..饲料中添加壳寡糖对斑马鱼肝肠健康和抗病能力的影响[J].中国农业科技导报,2025,27(5):222-230,9.基金项目
国家自然科学基金项目(32330110,32172991,32202959,32172958). （32330110,32172991,32202959,32172958）
