中国农业科技导报2025，Vol.27Issue(5)：231-238,8.DOI:10.13304/j.nykjdb.2024.0014
糙皮侧耳菌丝体液体发酵制备维生素D2条件的优化
Optimization of Preparation for Vitamin D2 by Liquid Fermentation of Pleutotus ostreatus Mycelium
摘要
Abstract
In order to obtain more vitamin D2(VD2)from Pleutotus ostreatus mycelium,VD2 was produced by liquid fermentation and photochemical transformation from Pleutotus ostreatus mycelium,and the process conditions were optimized.First,more ergosterol production was obtained from P.ostreatus mycelium by optimizing the liquid fermentation conditions.Then,Ultraviolet-B(UV-B,280～320 nm)irradiation was performed on the liquid fermentation of P.ostreatus mycelium.The effects of different UV intensity,irradiation area and irradiation time on the formation of VD2 were analyzed.The results revealed that the mycelium biomass was 3.23 g DW·L-1 and the yield of ergosterol reached 6.40 mg·L-1 under the fermentation temperature 28℃,the inoculation amount 5%,the shaker rate 120 r·min-1,glucose as carbon source,beef powder as nitrogen source,fermentation 5 d.When the UV intensity was 131.27 W·m-2,the irradiation area was 314.16 cm2,and the irradiation time was 80 min,the transformation rate of VD2 was 1.74%and the yield of VD2 was 111.24 µg·L-1,which the yield of VD2 increased by 37.55%compared with the before optimization.Above results provided data support for the development of VD2 process for the production of edible fungi.关键词
糙皮侧耳/菌丝体/UV-B/麦角甾醇/VD2Key words
Pleutotus ostreatus/mycelium/UV-B/ergosterol/VD2分类
园艺学与植物营养学引用本文复制引用
何孟欣,彭晴,徐小轻,田丹丹,弓雨欣,熊小艳,石波,乔宇..糙皮侧耳菌丝体液体发酵制备维生素D2条件的优化[J].中国农业科技导报,2025,27(5):231-238,8.基金项目
国家自然科学基金项目(32072773). （32072773）
