中国免疫学杂志2025，Vol.41Issue(5)：1030-1034,5.DOI:10.3969/j.issn.1000-484X.2025.05.002
ERMAP缺失加重IMQ诱导的小鼠银屑病样皮肤炎症
ERMAP deficiency aggravates IMQ-induced psoriasis-like skin inflammation in mice
摘要
Abstract
Objective:To investigate the effect of ERMAP on imiquimod(IMQ)-induced psoriasis-like skin inflammation in mice and its related mechanism.Methods:The experimental mice were divided into 3 groups:Sham group,WT group and ERMAP-/-group,with 9 mice in each group.The Sham group was smeared with Vaseline,and the WT group and ERMAP-/-group were smeared with IMQ to induce psoriatic dermatitis.The severity of IMQ-induced psoriasis lesions in mice were evaluated according to the psoria-sis area and severity index(PASI)and the HE staining pathology score.The expressions of F4/80 and Ki67 in mouse skin lesions were observed by immunofluorescence staining.The relative expressions of IL-1β,IL-6,IFN-γ and iNOS in skin lesions were detected by qRT-PCR.Flow cytometry was used to detect the proliferation and activation of T cells and the proportion of macrophages in spleen.Results:In the IMQ-induced mouse model of psoriasis-like dermatitis,the skin lesions of ERMAP gene knock out mice showed more severe squamous accumulation and skin bulge,more inflammatory cells aggregation and cytokine production,and the proportion of immune cells in the spleen of mice increased compared with the WT group,and the proportion of M1 macrophages increased.Conclu-sion:ERMAP deficiency aggravates IMQ-induced psoriasis-like skin inflammation in mice by enhancing immune response.关键词
ERMAP/银屑病/免疫反应/炎症因子Key words
ERMAP/Psoriasis/Immunoreaction/Inflammatory factors分类
中医学引用本文复制引用
夏露,陈伟,潘钇汶,刘智宏,苏敏..ERMAP缺失加重IMQ诱导的小鼠银屑病样皮肤炎症[J].中国免疫学杂志,2025,41(5):1030-1034,5.基金项目
国家自然科学基金项目(82171343,82060151) （82171343,82060151）
贵州省科学技术基金项目(黔科合支撑[2020]4Y230号,黔科合人才平台[2020]4103号). （黔科合支撑[2020]4Y230号,黔科合人才平台[2020]4103号）
