中国免疫学杂志2025，Vol.41Issue(5)：1035-1040,6.DOI:10.3969/j.issn.1000-484X.2025.05.003
miR-22-3p调控NF-κB信号通路对棕榈酸诱导的肝细胞炎症损伤和凋亡的影响
Effect of miR-22-3p regulation of NF-κB signaling pathway on palmitic acid-induced inflammation injury and apoptosis of hepatocytes
摘要
Abstract
Objective:To investigate the role and potential regulation mechanism of miR-22-3p in lipotoxic hepatocyte inflam-matory injury and apoptosis caused by palmitic acid(PA).Methods:Human normal immortalized hepatocytes(LO2 cells)were treated with different concentrations of PA for 24 h.CCK-8 and qRT-PCR were used to detect cell proliferation and miR-22-3p expression.miR-22-3p mimics or inhibitors were transfected into LO2 cells and then treated with 0.32 mmol/L PA for 24 h,the expression level of miR-22-3p was determined by qRT-PCR;cell viability was determined by CCK-8;biochemical kits to determine the ALT and AST contents;the mRNA and protein expression levels of inflammatory cytokines TNF-α,IL-1β and IL-6 in intracellular and culture super-natants were determined by qRT-PCR and ELISA;the cell apoptosis rate in each group was determined by flow cytometry;the expres-sion levels of NF-κB signaling pathway-related proteins were detected by Western blot and immunofluorescence.Results:With the increaseing of PA concentration,the cell survival rate and the expression of miR-22-3p decreased in a dose-dependent manner.After PA treatment,the cell proliferation activity decreased significantly,the activities of ALT and AST enzymes were increased,the expressions of inflammatory cytokines TNF-α,IL-1β and IL-6 were increased,cell apoptosis was increased,and NF-κB signaling pathway was activated.Transfection of miR-22-3p mimics significantly increased the proliferation activity of LO2 cells,and decreased the levels of ATL,AST,TNF-α,IL-1β,IL-6 and apoptosis,and inhibited the activation of NF-κB signaling pathway.Transfection of miR-22-3p inhibitor further activated NF-κB signaling pathway,and promoted cell inflammatory injury and apoptosis(all P<0.05).Conclusion:Overexpression of miR-22-3p can alleviate PA-induced apoptosis and inflammation,and the mechanism is related to inhibit the activation of NF-κB signaling pathway.关键词
miR-22-3p/脂毒性/肝细胞损伤/炎症/凋亡/NF-κB信号通路Key words
miR-22-3p/Lipid toxicity/Hepatocyte injury/Inflammation/Apoptosis/NF-κB signaling pathway分类
临床医学引用本文复制引用
许云春,于馨雅,李雨溦,郭乐..miR-22-3p调控NF-κB信号通路对棕榈酸诱导的肝细胞炎症损伤和凋亡的影响[J].中国免疫学杂志,2025,41(5):1035-1040,6.基金项目
国家自然科学基金(81960371) （81960371）
云南省科技厅科技计划项目(202001AU070014) （202001AU070014）
云南省教育厅科学研究基金项目(2023Y0951). （2023Y0951）
