中国免疫学杂志2025，Vol.41Issue(5)：1041-1047,7.DOI:10.3969/j.issn.1000-484X.2025.05.004
胶原诱导性关节炎大鼠血浆tRF和tiRNA的差异表达及功能分析
Differential expression and functional analysis of plasma tRF and tiRNA in collagen-induced arthritis rats
摘要
Abstract
Objective:To explore the differentially expressed tRNA-derived RNA fragment(tRF)and tRNA halves(tiRNA)in plasma of collagen-induced arthritis(CIA)rats,analyze the biological functions and related pathways of the tRF and tiRNAs target genes in order to identify new targets for diagnosis and therapy of rheumatoid arthritis(RA).Methods:Rat model of RA was estab-lished by injecting typeⅡ collagen.Arthritis index(AI)score and HE staining were conducted to evaluate the successfully establishment model.Illumina platform was used to obtain the tRF and tiRNA expression profiles.RT-qPCR was performed to validate expressions of tRF and tiRNA.Combining miRanda and TargetScan databases,the target genes of differentially expressed tRF and tiRNA were ob-tained.GO and KEGG enrichment analysis were used to annotate the functions of target genes.Results:A total of 25 tRFs and tiRNAs were significantly differentially expressed in plasma in rats after modeling,of which 23 were up-regulated and 2 were down-regulated.Besides,six specifically expressed tRFs and tiRNAs induced by CIA rats were screened,including Other-36:73-tRNA-Arg-CCT-4,Other-57:70-tRNA-Glu-CTC-1-M3,tRF-1:32-Gly-CCC-1-M2,tRF-1:31-Gly-CCC-1-M2,tRF-1:32-Pro-AGG-1-M4 and tRF-1:32-Glu-TTC-2-M2.GO analysis of target genes mainly involved organelle-associated items.What's more,KEGG analysis enriched multi-ple classical signal pathways associated with RA.Conclusion:Target genes of differentially expressed tRFs and tiRNAs in CIA rats may be involved in regulating important pathophysiological processes of RA and drug therapy targets.关键词
类风湿关节炎/胶原诱导性关节炎/转运RNA衍生片段/转运RNA半分子Key words
Rheumatoid arthritis/Collagen-induced arthritis/tRNA-derived RNA fragment/tRNA halves分类
临床医学引用本文复制引用
陈代奕,文宇琪,何彩林,胡恩,杨曌宇,熊新贵,杨波..胶原诱导性关节炎大鼠血浆tRF和tiRNA的差异表达及功能分析[J].中国免疫学杂志,2025,41(5):1041-1047,7.基金项目
国家自然科学基金(81874407) （81874407）
湖南省自然科学基金(2021JJ31117,2024JJ5566). （2021JJ31117,2024JJ5566）
