中国免疫学杂志2025，Vol.41Issue(5)：1048-1054,7.DOI:10.3969/j.issn.1000-484X.2025.05.005
PD-L2基因敲除小鼠的脾脏免疫表型分析
Immunophenotypic analysis of spleen in PD-L2 gene knockout mice
摘要
Abstract
Objective:To investigate impact of PD-L2 gene knockout on splenic immune phenotype of C57BL/6 mice.Methods:Body weight of female and male wild-type(WT)and PD-L2 gene knockout(KO)mice aged 3～12 weeks were continuously measured.Tissue structure differences in heart,liver,spleen,lung,kidney,thymus,ovary,uterus and testis of four groups of mice were observed by HE staining.Urease UV rate method was employed to measure urea level in mouse serum,and creatinine concentra-tion in serum was determined by creatine oxidase method.Flow cytometry was used to assess proportions of immune cells and cytokine differences in spleens of mice.Results:PD-L2 gene knockout had no apparent effect on body weight or organ structure of mice.Serum urea and creatinine levels were higher in female KO mice compared to WT mice,but no significant differences were observed among males.Female KO mice exhibited reduced proportions of CD4+T cells,CD8+T cells,Treg,NK cells and dendritic cells(DC)in spleen compared to WT mice.Conversely,proportions of Tfh,B cells,plasma cells,naïve B cells and B2 cells were elevated,proin-flammatory cytokines such as IL-17,IL-6 and IL-22 were increased,while anti-inflammatory cytokines like IL-10 and IL-4 were decreased.Differences among male mice were either not significant or absent.Conclusion:PD-L2 gene knockout primarily affects splenic immune phenotype of female mice,leading to increased inflammatory responses and weakened immune suppression after knockout.关键词
PD-L2/基因敲除/组织结构/免疫表型/炎症反应Key words
PD-L2/Gene knockout/Tissue structure/Immune phenotype/Inflammatory response分类
基础医学引用本文复制引用
杨艳红,宋赛哲,刘翠平..PD-L2基因敲除小鼠的脾脏免疫表型分析[J].中国免疫学杂志,2025,41(5):1048-1054,7.基金项目
苏州市姑苏卫生人才计划培养项目(GSWS2020011) （GSWS2020011）
苏州市科技计划项目(SKY2023138). （SKY2023138）
