中国免疫学杂志2025，Vol.41Issue(5)：1066-1071,6.DOI:10.3969/j.issn.1000-484X.2025.05.008
CD147对循环纤维细胞分化及促炎促纤维化功能的影响及机制研究
Effects and mechanisms of CD147 on differentiation of circulating fibrocytes and its pro-inflammatory and pro-fibrotic function
摘要
Abstract
Objective:To explore the effect and mechanism of CD147 on differentiation of circulating fibrocytes and its pro-inflammatory and pro-fibrotic function.Methods:Circulating fibrocytes were extracted from peripheral blood of asthma patients,stimu-lated with CD147,and intervened with CD147 antagonist AP-9 or p38/MAPK inhibitor SB203580.Cell viability was detected by CCK-8 method,cell migration was assessed by Transwell method,expressions of collagen type Ⅰ(COL-Ⅰ),CD45 and α-SMA were detected by immunofluorescence staining.Levels of cytokines such as TGF-β1,IL-4,IL-5 and IL-13 were detected by ELISA,and expression levels of COL-Ⅰ,fibronectin(FN),α-SMA,p-TAK1,p-P38 and other proteins were detected by Western blot.Results:Low dose of CD147 increased cell viability of circulating fibrocytes.CD147 significantly promoted migration of circulating fibrocytes and induces their differentiation into myofibrocytes.CD147 significantly increased secretion of cytokines such as TGF-β1,IL-4,IL-5 and IL-13 in circulating fibrocytes.CD147 significantly increased expressions of COL-Ⅰ,FN,α-SMA,p-TAK1,p-P38 in circulating fibrocytes.AP-9 or SB203580 significantly inhibited effect of CD147 on circulating fibrocytes.Conclusion:CD147 can promote proliferation,mi-gration,differentiation and pro-inflammatory and profibrotic function of circulating fibrocytes in vitro,and TAK1-p38/MAPK signaling pathway is involved in CD147-mediated regulation of circulating fibrocytes.关键词
CD147/气道重塑/循环纤维细胞/p38/MAPKKey words
CD147/Airway remodeling/Circulating fibrocytes/p38/MAPK分类
临床医学引用本文复制引用
周进才,李昭,程涛,林泽祥,王晶,韩新鹏..CD147对循环纤维细胞分化及促炎促纤维化功能的影响及机制研究[J].中国免疫学杂志,2025,41(5):1066-1071,6.基金项目
陕西省自然科学基础研究计划项目(2022JM-490). （2022JM-490）
