中国免疫学杂志2025，Vol.41Issue(5)：1122-1128,7.DOI:10.3969/j.issn.1000-484X.2025.05.017
小牛脾提取物对食管鳞癌术后辅助化疗患者的影响
Effects of calf spleen extract in patients with adjuvant chemotherapy in esophageal squamous carcinoma
刘爱芹 1闫冰 1任鹏 2蒋日成3
作者信息
- 1. 天津市肿瘤医院空港医院,国家恶性肿瘤临床医学研究中心,肿瘤精准转化诊疗科,天津 300000
- 2. 天津医科大学肿瘤医院食管肿瘤外科,国家肿瘤临床医学研究中心,天津市"肿瘤防治"重点实验室,天津市恶性肿瘤临床医学研究中心,天津 300000
- 3. 天津市肿瘤医院空港医院,国家恶性肿瘤临床医学研究中心,肿瘤精准转化诊疗科,天津 300000||天津医科大学肿瘤医院肺部肿瘤内科,国家肿瘤临床医学研究中心,天津市"肿瘤防治"重点实验室,天津市恶性肿瘤临床医学研究中心,天津 300000
- 折叠
摘要
Abstract
Objective:To investigate the effects of calf spleen extract(CSE)on immune function,serum ferritin levels,and adverse reactions in patients with esophageal squamous cell carcinoma undergoing adjuvant chemotherapy after surgery,and to verify its effects on esophageal cancer cell proliferation and apoptosis in vitro.Methods:A total of 96 postoperative chemotherapy patients with esophageal squamous cell carcinoma at Tianjin Cancer Hospital Airport Hospital were selected and randomly divided into control group and treatment group.They were treated with TP regimen as routine,and then treated with CSE in the treatment group.After the treatment was completed,adverse reactions and clinical reactions were observed in the two groups,and immune related indicators and ferritin levels were compared before and after treatment.Compared the disease free survival(DFS)differences between two groups of pa-tients during a follow-up period of 1 and 3 years.Using MTT assay to determine the IC50 value of CSE in esophageal cancer cells.Plate cloning method was used to detect the changes in the proliferation ability of esophageal squamous cell carcinoma cells induced by CSE.Comet assay detected DNA strand damage in esophageal cancer cells caused by CSE.Flow cytometry was used to detect the effect of CSE on apoptosis of esophageal cancer cells.Results:Comparison between the two groups of patients before and after treatment showed that CSE had a significant effect on CD3+T,CD4+T,CD8+T,CD4+T/CD8+T,Iron protein levels had an impact.CSE could alleviate the adverse reactions of postoperative chemotherapy in patients with esophageal cancer,and improve their quality of life and clinical symp-toms.CSE treatment could improve short-term DFS in postoperative patients with esophageal cancer.The IC50 concentration of CSE in esophageal cancer cells was 3.018 mg/ml;CSE could inhibit the proliferation of esophageal cancer cells and increase DNA strand dam-age in esophageal cancer cells.CSE can increase apoptosis of esophageal cancer cells.Conclusion:CSE can inhibit the proliferation of esophageal cancer cells,promote their apoptosis,and improve the quality of life and short-term DFS of esophageal cancer patients after treatment by regulating immune function and reducing adverse reactions.关键词
食管鳞癌/术后辅助化疗/免疫功能/铁蛋白Key words
Esophageal squamous cell carcinoma/Postoperative adjuvant chemotherapy/Immune function/Ferritin分类
医药卫生引用本文复制引用
刘爱芹,闫冰,任鹏,蒋日成..小牛脾提取物对食管鳞癌术后辅助化疗患者的影响[J].中国免疫学杂志,2025,41(5):1122-1128,7.
