中国免疫学杂志2025，Vol.41Issue(5)：1135-1139,5.DOI:10.3969/j.issn.1000-484X.2025.05.019
基于粪菌移植探讨黄芪多糖对脾虚水湿不化模型大鼠肠道免疫炎症的影响
Effect of Astragalus polysaccharides on intestinal immune inflammation of rats with dampness stagnancy due to spleen deficiency syndrome based on fecal microbiota transplantation
摘要
Abstract
Objective:To explore effect of Astragalus polysaccharides(APS)through TLR4/NF-κB signal pathway on intesti-nal immune inflammation of rats with dampness stagnancy due to spleen deficiency syndrome based on fecal microbiota transplantation.Methods:Rats with dampness stagnancy due to spleen deficiency syndrome were fed with high-fat and low-protein diet and subjected to exhaustive swimming,transplant flora microbiota after intervention of APS.General condition,changes in weight gain,time of weight exhaustion swimming,spleen index and thymus index were tested.Pathological manifestations of duodenal tissue inflammation were observed by HE staining.Serum IL-1β,IL-6 and TNF-α concentrations of rats were determined by ELISA.Protein levels of TLR4,NF-κB p65 and IκBα in colon were determined by Western blot.Results:Compared with model group,general condition rating,changes in weight gain and time of weight exhaustion swimming in APS-FTG group were increased significantly(P<0.01);spleen index and thymus index were increased;pathological manifestations of duodenal inflammation were significantly relieved;serum IL-1β,IL-6 and TNF-α concentrations were decreased significantly(P<0.01),TLR4 and NF-κB p65 levels in colon were decreased significantly(P<0.05 and P<0.01),IκBα level was in colon increased significantly(P<0.01).Conclusion:APS improves intestinal microbiota of rats with dampness stagnancy due to spleen deficiency syndrome and inhibits TLR4/NF-κB pathway activation through intestinal flora,thus improving intestinal immune inflammation status in rats with dampness stagnancy due to spleen deficiency syndrome.关键词
粪菌移植/黄芪多糖/TLR4/NF-κB通路/脾虚水湿不化/肠道菌群/免疫炎症Key words
Fecal microbiota transplantation/Astragalus polysaccharides/TLR4/NF-κB pathway/Dampness stagnancy due to spleen deficiency syndrome/Intestinal flora/Immune inflammation分类
医药卫生引用本文复制引用
郑俊,段晨晨,吕琴,赵雪莲,杨彬彬,王世军,赵文晓..基于粪菌移植探讨黄芪多糖对脾虚水湿不化模型大鼠肠道免疫炎症的影响[J].中国免疫学杂志,2025,41(5):1135-1139,5.基金项目
国家自然科学基金(81903947,81803864) （81903947,81803864）
山东省重点研发计划项目(2019GSF108209). （2019GSF108209）
