中国标准化Issue(8)：155-158,4.DOI:10.3969/j.issn.1002-5944.2025.08.026
柱前衍生-HPLC法测定桑枝总生物碱含量
Determination of Total Alkaloids in Mulberry Branch by Pre-column Derivatization and HPLC
余为 1秦德松 1唐雪梅 1李月平 1周春红1
作者信息
摘要
Abstract
Objective:To establish a pre-column derivatization and high performance liquid phase method for the determination of total alkaloids in mulberry plants.Methods:Samples were collected with water and injected with 9-chloroformate methyl fluorene derivative reagent by HPLC with the volume of 20 µL.The chromatographic analysis was performed at 32℃with red spectrum RDC18(5 μm,4.6 x 250 mm)on the column and gradient elution of(citric acid buffer pH 4.2)(A)-acetonitrile(B)(75%B→47.5%B at 0～22 min,47.5%B→37.5%B at 22～22.01 min,37.5%B→37.5%B at 22.01～38 min,37.5%B→75%B at 38～38.01 min,75%B→75%B at 38.01～43 min).The flow rate was 1.5 mL/min.Results:In the linear range of 5～500 ug/mL,the linear relationship was good(r≥0.99),and the detection limit was 6.28 mg.kg-1.Conclusion:The method is rapid,simple and sensitive,and can be used for the determination of total alkaloid content in mulberry branch.关键词
桑枝/桑植总生物碱/含量测定/抗真菌类/柱前衍生/RP-HPLCKey words
mulberry branch/total alkaloids of mulberry plant/content determination/antifungal/pre-column derivation/RP-HPLC引用本文复制引用
余为,秦德松,唐雪梅,李月平,周春红..柱前衍生-HPLC法测定桑枝总生物碱含量[J].中国标准化,2025,(8):155-158,4.
