中国免疫学杂志2025，Vol.41Issue(5)：1202-1207,6.DOI:10.3969/j.issn.1000-484X.2025.05.030
基于多重微球流式免疫荧光法检测成人血浆12种细胞因子参考区间的初步建立
Preliminary establishment of reference intervals for 12 cytokines in adult plasma by multiplex bead-based flow fluorescent immunoassay
摘要
Abstract
Objective:To establish the reference interval of 12 types of cytokines(IL-1β,IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12p70,IL-17,IFN-γ,IFN-α,TNF-α)in adult plasma based on multiple microsphere flow immunofluorescence(MBFFI).Methods:A total of 140 healthy adult patients who were examined at Xuzhou Central Hospital between January 2022 and December 2023 were included in the study.Plasma cytokine levels were detected and reference intervals were established by the flow cytometer and the assay kits produced by Qingdao Raisecare Biotechnology Co.,Ltd and Jiangsu BioPredia Biotechnology Co.,Ltd.Results:All of the cytokines exhibited a non-normal distribution,and there was a discrepancy in the 95％reference interval between the two re-agents.The reference intervals for the 12 cytokine kits produced by Qingdao Raisecare Biotechnology Co.,Ltd.were as follows:IFN-α:<4.91 pg/ml,IL-12 p70:<1.95 pg/ml,IL-5:<12.72 pg/ml,IL-8:<60.68 pg/ml,IL-1β:<27.67 pg/ml,IL-2:<5.01 pg/ml,IL-4:<1.22 pg/ml,IL-6:<6.11 pg/ml,TNF-α:<2.92 pg/ml,IL-17:<10.27 pg/ml,IL-10:<6.88 pg/ml,IFN-γ:<17.68 pg/ml.The reference intervals of the 12 cytokines produced by Jiangsu BioPredia Biotechnology Co.,Ltd.were as follows:IFN-α:<4.05 pg/ml,IL-12 p70:<7.33 pg/ml,IL-5:<7.80 pg/ml,IL-8:<13.24 pg/ml,IL-1β:<19.24 pg/ml,IL-2:<2.42 pg/ml,IL-4:<0.99 pg/ml,IL-6:<2.10 pg/ml,TNF-α:<0.87 pg/ml,IL-17:<1.42 pg/ml,IL-10:<1.10 pg/ml,IFN-γ:<1.34 pg/ml.Conclusion:In this study,the ref-erence range of two reagents for the detection of 12 kinds of cytokines in plasma of healthy adults is established by MBFFI,which pro-vides a valuable reference for the diagnosis and treatment of clinical-related diseases.关键词
多重微球流式免疫荧光法/细胞因子/参考区间Key words
Multiplex bead-based flow fluorescent immunoassay/Cytokines/Reference interval分类
医药卫生引用本文复制引用
王新宇,程星,郑璐,张玥,马雨婷,牛国平,顾锋,陈永强..基于多重微球流式免疫荧光法检测成人血浆12种细胞因子参考区间的初步建立[J].中国免疫学杂志,2025,41(5):1202-1207,6.基金项目
徐州市卫健委"彭城英才-医学青年后备人才"项目(XWRCHT20220006). （XWRCHT20220006）
