中国免疫学杂志2025，Vol.41Issue(5)：1215-1219,5.DOI:10.3969/j.issn.1000-484X.2025.05.032
兔抗人源KHSRP-Acetyl-K205多克隆抗体制备、鉴定及临床意义
Generation,identification and clinical significance of rabbit polyclonal anti-body against human KHSRP-Acetyl-K205
王磊 1蔡人杰 2田广玉 1徐明2
作者信息
- 1. 扬州大学附属江都人民医院肿瘤科,扬州 225200
- 2. 上海交通大学医学院附属第九人民医院肿瘤科,上海 201999
- 折叠
摘要
Abstract
Objective:To generate a rabbit polyclonal antibody specific for human acetylated KHSRP and to evaluate its poten-tial value and clinical significance in detecting colorectal cancer samples.Methods:An acetylated peptide Lys205(K205)of KHSRP protein was designed and synthesized,which was coupled to KLH at its carboxyl terminus to form an antigen-carrier conjugate.After five times immunization in New Zealand rabbits,blood serum was separated to determine antibody titer by indirect ELISA.Polyclonal antibody by affinity chromatography method was purified,and specificity of antibody was determined by Western blot and immunohis-tochemistry(IHC).Results:Final rabbit serum antibody ELISA titer was>5.12×105,which could be used for specific detection of K205-acetylated KHSRP protein by Western blot and IHC.Conclusion:Role of KHSRP in tumor may be closely related to its surface acetylation modification.A rabbit polyclonal antibody against human acetylated KHSRP has been successfully generated,and an excel-lent foundation for assessing relationship between acetylated KHSRP and tumor processes in cancer diseases has been made.关键词
KHSRP/乙酰化/兔多克隆抗体/肿瘤Key words
KHSRP/Acetylation/Rabbit polyclonal antibody/Tumor分类
医药卫生引用本文复制引用
王磊,蔡人杰,田广玉,徐明..兔抗人源KHSRP-Acetyl-K205多克隆抗体制备、鉴定及临床意义[J].中国免疫学杂志,2025,41(5):1215-1219,5.
