中国药理学通报2025，Vol.41Issue(5)：970-978,9.DOI:10.12360/CPB202407100
基于UHPLC-Q-Orbitrap MS技术的仙鹤草干预H1299细胞增殖和凋亡的代谢组学分析
Metabolomic analysis of Agrimonia pilosa intervention in proliferation and apoptosis of H1299 cells based on UHPLC-Q-Orbitrap MS technology
摘要
Abstract
Aim To investigate the effects of Agrimonia pilosa(AP)on the proliferation and apoptosis of non-small cell lung cancer(NSCLC)H1299 cells using non-targeted metabolomics and other methods,and to explore the underlying molecular mechanisms.Meth-ods Taking H1299 cells as the research object,the effect of AP on cell proliferation and apoptosis was de-tected through CCK-8 method,colony formation,LDH,Hoechst 33258 staining,AO/EB staining,flow cytometry detection,RT qPCR and other experiments.The main differential metabolites were detected by the metabolomics method of ultra-high phase liquid chro-matography and mass spectrometry(UHPLC-Q-Orbi-trap MS),and related metabolic pathways were ana-lyzed.Results Compared with the control group,AP treatment was able to significantly inhibit the prolifera-tion and colony formation of H1299 cells,while the re-lease of LDH increased in a dose-dependent manner.Fluorescence microscopy and flow cytometry and RT-qPCR analysis revealed that H1299 cells underwent crumpling and increased nuclear fragmentation after AP administration,blocked in G0/G1 phase,up-regulated apoptotic genes caspase-3 and Bax,and down-regulated apoptosis-inducing effects of Bcl-2.Metabolomics anal-ysis screened 35 differential metabolites,which were PC(O-30∶1),D-Glutamic acid,PE(18∶0/15∶0),etc.The main metabolic pathways involved includ-ed amino acid metabolism,glycerophospholipid metabo-lism and purine metabolism so on.Conclusions AP may exert its pharmacological effects by interfering with multiple metabolic pathways in H1299 cells,inhibiting cell proliferation and promoting apoptosis.关键词
仙鹤草/H1299细胞/代谢组学/细胞凋亡/非小细胞肺癌/细胞增殖Key words
Agrimonia pilosa/H1299 cells/metabolo-mics/cell apoptosis/NSCLC/cell proliferation分类
医药卫生引用本文复制引用
同泽华,郭文军,李萌,徐雅娟,张洪铭,窦泽宇,解生旭,王卫芳..基于UHPLC-Q-Orbitrap MS技术的仙鹤草干预H1299细胞增殖和凋亡的代谢组学分析[J].中国药理学通报,2025,41(5):970-978,9.基金项目
吉林省科技发展计划项目(No YDZJ202201ZYTS181) （No YDZJ202201ZYTS181）
吉林省教育厅科学研究项目(No JJKH20241084KJ) （No JJKH20241084KJ）
吉林省中医药管理局科研项目(No 2020053) （No 2020053）
吉林省博士后科研资助项目(No 201410) （No 201410）
