中国药理学通报2025，Vol.41Issue(5)：978-984,7.DOI:10.12360/CPB202409069
基于PI3K/AKT/NF-κB信号通路探讨清解化攻方减轻急性胰腺炎炎症反应的作用机制
Mechanism of action of Qingjie Huagong decoction reducing inflammatory response of acute pancreatitis based on PI3K/AKT/NF-κB signaling pathway
摘要
Abstract
Aim To explore the therapeutic effect and mechanism of Qingjie Huagong decoction in modulating PI3K/AKT/NF-κB signaling pathway in inflammatory response of acute pancreatitis(AP)mice.Methods Twenty-four mice were randomly divided into Blank group,Model group,Ustekin group,and Qingjie Hua-gong decoction group,with six mice in each group.The AP model was prepared by using rain frogin.Serum α-AMS,PNLP,IL-1β,IL-6,IL-8,IL-18,and TNF-α lev-els were detected by ELISA;the pancreatic pathology was detected by HE staining;the expressions of PI3K,AKT,and NF-κB-related proteins and mRNAs were de-tected by immunohistochemistry,Western blot,and RT-qPCR.Results Compared with the blank group,the model group showed obvious pathological damage to the pancreas,with significantly higher serum α-AMS,PN-LP,IL-1β,IL-6,IL-8,IL-18,and TNF-α levels(P＜0.01),and significantly higher levels of PI3K,AKT,and NF-κB-related proteins and mRNA expression(P＜0.01).Compared with the model group,both the Qingjie Huagong decoction group and the ustekin group improved the histopathological changes in the pancreas of AP mice,decreased the serum α-AMS,PNLP,IL-1β,IL-6,IL-8,IL-18,and TNF-α levels,and down-reg-ulated the expression levels of pancreatic PI3K,AKT,NF-κB-related proteins and mRNA(P＜0.05 or P＜0.01).Conclusion Qingjie Huagong decoction may inhibit the inflammatory response and protect pancreat-ic tissues by regulating the expression of PI3K/AKT/NF-κB signaling pathway.关键词
清解化攻方/PI3K/AKT/NF-κB信号通路/急性胰腺炎/炎症反应/胰腺/作用机制Key words
Qingjie Huagong decoction/PI3K/AKT/NF-κB signaling pathway/acute pancreatitis/inflam-matory response/pancreas/mechanism of action分类
医药卫生引用本文复制引用
朱晓东,陈国忠,冯敏超,刘锟荣,班莹,苏攀,禤传凤,黄小艺,李德文,唐曦平..基于PI3K/AKT/NF-κB信号通路探讨清解化攻方减轻急性胰腺炎炎症反应的作用机制[J].中国药理学通报,2025,41(5):978-984,7.基金项目
国家自然科学基金资助项目(No 82160890) （No 82160890）
广西自然科学基金面上项目(No2024GXNSFAA010246) （No2024GXNSFAA010246）
国家中医优势专科建设项目(No 2024010) （No 2024010）
广西中医药大学引进博士科研启动基金项目(No 2022BS029) （No 2022BS029）
"十四五"防城港市科技计划项目(No 防科AB23006034) （No 防科AB23006034）
