中国药理学通报2025，Vol.41Issue(5)：985-993,9.DOI:10.12360/CPB202409059
真武汤对脂多糖诱导的肾小球上皮细胞炎症、氧化应激及凋亡的影响
Effects of Zhenwu decoction on inflammation,oxidative stress,and apoptosis in glomerular epithelial cells induced by lipopolysaccharide
摘要
Abstract
Aim To investigate the effect of Zhenwu decoction on inflammation,oxidative stress and apopto-sis of human glomerular epithelial cells(HGEC)in-duced by lipopolysaccharide(LPS)based on Nrf2/HO-1 signaling pathway,and to explore the underlying mechanism.Methods HGEC were treated with LPS(1.0 mg·L-1)for 24 h to construct an oxidative damage model.On this basis,2.5％,5％and 10％Zhenwu decoction-containing serum were added to the low,medium and high dose groups of Zhenwu decoc-tion,and a normal group was set up.The changes of cell activity were assessed by MTT method and LDH method.The contents of TNF-α,IL-6,IL-10,SOD,CAT,GSH-Px,ROS and MDA in each group were de-tected by ELISA.The apoptosis of each group was de-tected by flow cytometry.The mRNA and protein ex-pressions of Bax,Bcl-2,caspase-3,caspase-9 and Nrf2/HO-1 pathway were detected by RT-qPCR and Western blot,respectively.Results Compared to the normal group,the model group of HGEC exhibited increased levels of inflammatory cytokines,enhanced oxidative stress response and aggravated apoptosis;after inter-vention with various doses of Zhenwu decoction,the in-flammatory levels in HGEC were reduced,oxidative damage and apoptosis were effectively ameliorated,and the mRNA and protein expression levels of the Nrf2/HO-1 signaling pathway were upregulated.Conclu-sions Zhenwu decoction can protect HGEC from LPS-induced inflammation and oxidative damage and im-prove apoptosis.The mechanism may be related to the activation of Nrf2/HO-1 signaling pathway.关键词
真武汤/肾小球上皮细胞/脂多糖/炎症/氧化应激/凋亡/Nrf2/HO-1信号通路Key words
Zhenwu decoction/glomerular epithelial cells/lipopolysaccharides/inflammation/oxidative stress/apoptosis/Nrf2/HO-1 signaling pathway分类
医药卫生引用本文复制引用
王曼非,柴希,高夏夏,储开博,张育敏,田岳凤,何丽清..真武汤对脂多糖诱导的肾小球上皮细胞炎症、氧化应激及凋亡的影响[J].中国药理学通报,2025,41(5):985-993,9.基金项目
国家自然科学基金面上项目(No 82274365) （No 82274365）
山西省自然科学基金面上项目(No 20210302123228) （No 20210302123228）
山西中医药大学科技创新团队(No 2022TD1011) （No 2022TD1011）
