中国药理学通报2025，Vol.41Issue(5)：994-999,6.DOI:10.12360/CPB202410081
神经元与小胶质细胞示踪及可清除小鼠模型构建
Construction of a mouse model to trace neurons and microglia and ablate microglia for investigating their interactive roles
摘要
Abstract
Aim To generate a dual-reporter mouse model for tracing microglia and neurons to analyze their dynamic changes in real-time and to investigate the functional role of microglia-neuron interactions through microglial depletion.Methods Mi-croglia reporter mice were crossed with neuron reporter mice to generate Tmem119-e(2A-tdTomato-2A-DTR);Thy1-GFP dual-reporter mice.Genotyping was performed using tail DNA.Con-focal and two-photon microscopy were used to examine neuronal morphology.In vivo two-photon imaging was employed to observe the distribution of microglia and neurons in the brain.Diphtheria toxin(DT)was intraperitoneally injected to deplete microglia.Results The dual-reporter mice were successfully generated with the correct genotype.Both microglia and neurons were visu-alized,and microglia were specifically depleted by DT.Conclu-sions The successful establishment of a dual-reporter mouse model for tracing neurons and microglia will facilitate real-time in vivo observation of microglial and neuronal changes under physiological or pathological conditions,elucidating their specific roles in brain homeostasis.关键词
小胶质细胞/神经元/脑稳态/活体可视化/双光子成像/中枢神经系统疾病Key words
microglia/neurons/brain homeostasis/in vivo visu-alization/two-photon imaging/central nervous system diseases分类
医药卫生引用本文复制引用
陈梅,张准宏,楚世峰,张钊,陈乃宏..神经元与小胶质细胞示踪及可清除小鼠模型构建[J].中国药理学通报,2025,41(5):994-999,6.基金项目
国家自然科学基金资助项目(No 82374060) （No 82374060）
中国医学科学院医学与健康科技创新工程项目(No 2021-I2M-1-020) （No 2021-I2M-1-020）
