中国药房2025，Vol.36Issue(9)：1040-1045,6.DOI:10.6039/j.issn.1001-0408.2025.09.04
基于定性、定量及化学模式识别分析的蒙药森登-4质量评价
Quality evaluation of Mongolian medicine Sendeng-4 based on qualitative and quantitative analysis combined with chemical pattern recognition
摘要
Abstract
OBJECTIVE To evaluate the quality of Mongolian medicine Sendeng-4 based on qualitative and quantitative analysis combined with chemical pattern recognition,in order to provide the reference for its quality control.METHODS The chemical components in Sendeng-4 were analyzed qualitatively by HPLC-Q-Exactive-MS.The contents of 16 components(methyl gallate,ethyl gallate,epicatechin,dihydromyricetin,genipin-1-O-β-D-gentiobioside,caffeic acid,catechin,corilagin,deacetylasperulosidic acid methyl ester,rutin,geniposide,luteolin,myricetin,quercetin,ferulic acid,and toosendanin)in 15 batches of Sendeng-4(sample S1-S15)were quantitatively analyzed by HPLC-MS/MS.Cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least squares discriminant analysis were conducted and variable importance projection(VIP)value greater than 1 was used as the index to screen the differential components.RESULTS A total of 73 chemical components were identified in Sendeng-4,including 20 flavonoids,16 tannins,14 organic acids,etc.According to the quantitative analysis,the results exhibited that the average contentsthe of above 16 components in 15 batches of Sendeng-4 were 3.683-7.730,2.391-6.952,2 275.538-4 377.491,2 699.188-3 537.924,858.266-1 377.393,3.366-11.003,140.624-315.683,414.629-978.334,285.501-1 510.457,27.799-48.325,3 625.415-6 309.563,0.506-0.656,442.337-649.283,47.093-59.736,12.942-15.822,127.738-326.649 μg/g,respectively.According to the results of CA and PCA,15 batches of samples could be clustered into two categories:S1-S3,S5-S6,S9-S10 and S13 were clustered into one category;S4,S7-S8,S11-S12,S14-S15 were clustered into one category.VIP values of geniposide,epicatechin,deacetylasperulosidic acid methyl ester and genipin-1-O-β-D-gentiobioside were all greater than 1.CONCLUSIONS HPLC-Q-Exactive-MS and HPLC-MS/MS techniques are employed for the qualitative and quantitative analysis of Sendeng-4.Through chemical pattern recognition analysis,four differential components are identified:geniposide,epicatechin,deacetylasperulosidic acid methyl ester,and genipin-1-O-β-D-gentiobioside.关键词
森登-4/定性分析/定量分析/化学模式识别分析/含量测定/质量评价/差异性成分Key words
Sendeng-4/qualitative analysis/quantitative analysis/chemical pattern recognition analysis/content determination/quality evaluation/differential components分类
药学引用本文复制引用
周枫叶,李君,张谦,李荣杰,张微,刘静,王芳,李胜男..基于定性、定量及化学模式识别分析的蒙药森登-4质量评价[J].中国药房,2025,36(9):1040-1045,6.基金项目
国家自然科学基金项目(No.82460822,No.82360801) （No.82460822,No.82360801）
内蒙古自治区自然科学基金项目(No.2024QN08054,No.2023QN08012) （No.2024QN08054,No.2023QN08012）
内蒙古自治区卫生健康科技计划项目(No.202201187) （No.202201187）
内蒙古医科大学联合项目(No.YKD2024LH005,No.YKD2023LH062,No.YKD2022LH031) （No.YKD2024LH005,No.YKD2023LH062,No.YKD2022LH031）
内蒙古医学科学院公立医院科研联合基金科技项目(No.2024GLLH0329) （No.2024GLLH0329）
