中华中医药杂志2025，Vol.40Issue(4)：1679-1686,8.
补脾养心方对心脾两虚亚健康失眠小鼠睡眠改善的机制
Mechanism of Bupi Yangxin Decoction on improving sleep of sub-health insomnia mice with heart and spleen deficiency
摘要
Abstract
Objective:To explore effect and underlying mechanism of Bupi Yangxin Decoction on improving sleep of sub-health insomnia mice with heart and spleen deficiency.Methods:The sub-healthy insomnia mice model with heart and spleen deficiency was duplicated by swim with exhaustion load combined with gavage of propranolol hydrochloride solution.Sixty male Kunming mice were randomly divided into 6 groups(n=10):blank control group,model group,positive control group(Renshen Guipi Pills 5 g/kg)and Bupi Yangxin Decoction low-,medium-,and high-dose groups(1.5,3,6 g/kg),continuous administration for 30 days.The direct sleep test,pentobarbital sodium sleep time extension test,barbital sodium sleep latency test,pentobarbital sodium subthreshold hypnosis test and HE staining of brain tissue were adopted to evaluate the sleep function of mice in each group.The ELISA and Western Blot method were used to detect protein concentrations and expressions related to sleep mechanism of mice.Results:Compared with the model group,Bupi Yangxin Decoction medium-and high-dose groups could lengthen the sleep time of pentobarbital sodium,shorten the sleep latency of barbital sodium,and increase the incidence of subthreshold sleep induced by pentobarbital sodium(P＜0.01,P＜0.05);improve inflammatory changes in brain tissue;up-regulate GABRA1,GAD1,BDNF proteins and down-regulate the expressions of TNF-α and dopamine D1 receptor protein(P＜0.01,P＜0.05);increase serum GABA and decrease Glu content and the Glu/GABA ratio(P＜0.01);increase the monoamine neurotransmitter 5-HT in the serum of mice,and decrease the content of DA and NE(P＜0.01).Conclusion:Bupi Yangxin Decoction can improve sleep of sub-health insomnia mice with heart and spleen deficiency.The mechanism may be related to regulating the sleep-wake cycle,regulating the content of monoamine neurotransmitters and reducing inflammation.关键词
改善睡眠/补脾养心方/睡眠-觉醒周期/单胺类神经递质/炎症/失眠/亚健康Key words
Improving sleep/Bupi Yangxin Decoction/Sleep-wake cycle/Monoamine neurotransmitter/Inflammation/Insomnia/Sub-health引用本文复制引用
廖艳,热依拉·吐尔逊,刘冰清,乐娜,高鑫,林殷..补脾养心方对心脾两虚亚健康失眠小鼠睡眠改善的机制[J].中华中医药杂志,2025,40(4):1679-1686,8.基金项目
国家重点研发计划(No.2018YFC1706800) （No.2018YFC1706800）
