中国药理学通报2025，Vol.41Issue(5)：837-843,7.DOI:10.12360/CPB202409051
基于单细胞测序探讨金丝桃素抗抑郁的作用机制
Exploring mechanism of action of hypericin in antidepressant effects based on single-cell sequencing
摘要
Abstract
Aim To investigate the antidepressant mechanism of hyperforin via the utilization of single-cell sequencing technology.Methods C57BL/6 mice were randomly divided into the control group,depres-sion model group,and hyperforin intervention group.The chronic unpredictable mild stress(CUMS)model was induced and drug interventions were administered for 28 d.Behavioral experiments were conducted to as-sess depressive symptoms,and hippocampal tissue was collected for single-cell RNA sequencing.Key cell populations and differentially expressed genes across groups were identified,followed by PPI network,GO,and KEGG enrichment analysis.Results Behavioral experiments indicated that CUMS successfully induced depressive symptoms in mice,while hyperforin im-proved depressive behavior.In the depression model group,the proportion of brain perivascular macrophages(PVM)increased,and this proportion decreased after hyperforin intervention,approaching the level seen in the control group.The top 20 common differentially ex-pressed genes in the PVM subpopulation were Saa3,Hbb-bs and Ccl24.PPI network analysis identified core targets,including Ccl2,Dhx9,C3,Msr1,Cxcl2 and Cx3cr1.KEGG enrichment analysis revealed pathways related to chemokines,phagosome formation,and inosi-tol phosphate metabolism.Conclusion The antide-pressant mechanism of hyperforin may be related to the regulation of Ccl24 and its related chemokine signaling pathway by PVM.关键词
抑郁症/单细胞测序/金丝桃素/脑血管周围巨噬细胞/Ccl24/趋化因子信号通路Key words
depression/single-cell sequencing/hyper-forin/cerebral perivascular macrophages/Ccl24/che-mokine signaling pathway引用本文复制引用
倪慧心,许茜,刘海鑫,周炳灿,陈明恒,林萍燕,高正涛,林新培,林瑶,吴方真..基于单细胞测序探讨金丝桃素抗抑郁的作用机制[J].中国药理学通报,2025,41(5):837-843,7.基金项目
国家自然科学基金资助项目(No 81904263) （No 81904263）
福建省自然科学基金资助项目(No 2024J01745,2024J01123) （No 2024J01745,2024J01123）
福建中医药大学中医健康管理学联合省级临床重点专科建设项目(老年病科)(No XJG2023018) （老年病科）
福建中医药大学校管课题青年项目(No X2023010) （No X2023010）
