中国现代医学杂志2025，Vol.35Issue(9)：46-53,8.DOI:10.3969/j.issn.1005-8982.2025.09.008
S1P对成肌细胞移植治疗心肌梗死后心律失常的影响及其机制探讨
The effect and mechanism of S1P reduces arrhythmia after myoblast transplantation for myocardial infarction
摘要
Abstract
Objective To explore the effect and mechanism of sphingosine-1-phosphate(S1P)on the development of arrhythmia after autologous myoblasts transplantation.Methods Arrhythmia was induced by procedural stimulation,and Ca2+concentration was measured by Ca2+-ATPase activity in myocardial tissue.Rat cardiomyocytes were isolated and cultured in vitro,and co-cultured with skeletal muscle myoblasts to construct a hypoxia/reoxygenation injury model.S1P intervention was started with the reoxygenation phase,and the protein expression of Connexin43(Cx43)was detected by Western blotting and immunohistochemistry.Thus,to clarify the effect of S1P on arrhythmia after myoblast transplantation for myocardial infarction,and initially explore the mechanism of S1P in reducing arrhythmia after cell transplantation.Results The onset of arrhythmia in the S1P cell transplant group was not only delayed,but the induction rate was decreased,and the duration was shorter than that in the control group(P<0.05).Ca2+-ATPase activity was higher in cells transplanted into S1P than in cells transplanted alone(P<0.05).In the hypoxia/reoxygenation injury model of isolated myoblasts and cardiomyocytes,Cx43 protein expression increased significantly with increasing S1P(P<0.05).Conclusions S1P promotes the development of Ca2+-ATPase activity and gap link Cx43 protein through myocardial ischemia and reperfusion,thus reducing the occurrence of ventricular arrhythmia after cell transplantation.关键词
心肌梗死/心律失常/细胞移植/1-磷酸鞘氨醇/连接蛋白43Key words
myocardial infarction/arrhythmia/cell transplantation/sphingosine-1-phosphate/connexin43分类
临床医学引用本文复制引用
于欢,许晓欣,张伊..S1P对成肌细胞移植治疗心肌梗死后心律失常的影响及其机制探讨[J].中国现代医学杂志,2025,35(9):46-53,8.基金项目
国家自然科学基金(No:81760263) （No:81760263）
福建省自然科学基金(No:2022J011411) （No:2022J011411）
福建省教育厅中青年教师教育科研项目(No:JAT200734) （No:JAT200734）
2021年厦门市留学人员科研项目(No:LX202101) （No:LX202101）
