中国药房2025，Vol.36Issue(9)：1059-1064,6.DOI:10.6039/j.issn.1001-0408.2025.09.07
双术汤对大鼠的安全性及改善消化性不良的药效作用研究
Study on the safety and pharmacological effect on improving dyspepsia of Shuangshu decoction in rats
摘要
Abstract
OBJECTIVE To study the safety of Shuangshu decoction in rats and its efficacy in improving functional dyspepsia(FD)in rats.METHODS In safety test,40 rats were divided into blank control group,Shuangshu decoction low-dose,medium-dose and high-dose groups[108,216,324 g/(kg·d),calculated by raw medicine,the same applies below];they were given relevant medicine intragastrically,for continuous 14 days.The mortality and toxic reactions of rats were recorded,and the organ indexes of the liver,kidney,spleen,lung and heart of rats were calculated;the pathological morphological changes in the liver,kidney,spleen,lung,heart,stomach,duodenum,and colon were observed to evaluate the acute toxicity of Shuangshu decoction.Another 40 rats were grouped and administered in the same way for 30 consecutive days.The mortality and toxic reactions of the rats were recorded,and the corresponding organ indexes were calculated.The pathological morphological changes in the corresponding organs were observed,and blood routine and serum biochemical indicators were measured,in order to assess the subacute toxicity of Shuangshu decoction.In pharmacodynamic experiments:50 rats were divided into blank control group,model group,and Shuangshu decoction low-,medium-,and high-dose groups(9.45,18.9,37.8 g/kg),with 10 rats in each group.Except for blank control group,rats in all other groups were used to establish the FD rat model by subcutaneous injection of loperamide(3.5 mg/kg).Rats in each group were administered the corresponding drug solution/normal saline intragastrically,once a day,for 14 consecutive days.After the last medication,fecal moisture content,intestinal propulsion rate,gastric emptying rate and serum level of motilin were all detected,and interstitial cell of Cajal(ICC)ultrastructure of rats was observed in colon tissue.RESULTS The safety experiments showed that no death occurred in each dose group,and no significant difference was found in organ coefficient,routine blood and serum biological index,compared to blank control group(P＞0.05);no abnormality was found in organ appearance and pathological sections.The results of the pharmacodynamic experiments showed that,compared with the blank control group,the fecal moisture content,gastric emptying rate,intestinal propulsion rate,and serum motilin levels in the model group were significantly decreased(P＜0.05);in the colonic tissue,the mitochondria in the ICC exhibited severe swelling with the disappearance of cristae,and the endoplasmic reticulum was dilated.Compared with model group,the rats in Shuangshu decoction high-dose group showed significant increases in the above quantitative indicators(P＜0.05);additionally,there was a large number of mitochondria in the ICC of the colonic tissue,with clear cristae and regular arrangement.CONCLUSIONS Shuangshu decoction is safe and has a beneficial improving effect on FD rats;its mechanism of action may be related to the regulation of gastrointestinal hormone expression to promote gastric emptying and intestinal propulsion,as well as the repair of mitochondrial structure in ICCs to restore gastrointestinal function.关键词
双术汤/安全性/药效学/急性毒性/亚急性毒性/消化性功能不良/胃肠动力障碍Key words
Shuangshu decoction/safety/pharmacodynamics/acute toxicity/subacute toxicity/functional dyspepsia/gastrointestinal motility disorder分类
药学引用本文复制引用
陈鑫源,林才志,熊常州,李炯汾,余康怡,徐欢,王樱遐,廖丹,陶俊宇,杨子仪..双术汤对大鼠的安全性及改善消化性不良的药效作用研究[J].中国药房,2025,36(9):1059-1064,6.基金项目
国家自然科学基金项目(No.82060834) （No.82060834）
