中国药理学通报2025，Vol.41Issue(5)：861-867,7.DOI:10.12360/CPB202412017
白藜芦醇通过Nrf2/Keap1途径减轻类风湿关节炎肝脏炎症和氧化应激
Resveratrol attenuates hepatic inflammation and oxidative stress in rheumatoid arthritis via Nrf2/Keap1 pathway
摘要
Abstract
Aim To explore the therapeutic effects of resveratrol(Res)on hepatic inflammation and oxida-tive stress in rheumatoid arthritis(RA),and to eluci-date the relationship of the regulatory mechanism of the Nrf2/Keap1 signaling pathway in it.Methods A mouse model of arthritis was induced using chicken type Ⅱ collagen in combination with complete Freund's adjuvant,and Res was administered by tube feeding for treatment.Serum liver function indices and levels of hepatic inflammation and oxidative stress were detected in mice.An in vitro cellular model of hepatic inflam-mation and oxidative stress was established by treating mouse primary hepatocytes(MPHs)with TNF-α(5μg·L-1),cell proliferation inhibition was detected by CCK-8,and inflammation and oxidative stress-relat-ed indices were detected by protein blotting.The in-trinsic mechanisms by which Res attenuated hepatic in-flammation and oxidative stress in rheumatoid arthritis were explored by treating MPHs with Nrf2 inhibitor and Keap1 overexpression plasmid.Results Res signifi-cantly reduced the levels of inflammation and oxidative stress in hepatic tissues of collagen-induced arthritis mice as well as TNF-α-treated MPHs,and activated the Nrf2/Keap1 signaling pathway.Inflammation and oxidative stress levels in MPHs were exacerbated by the use of Nrf2 inhibitors and Keap1 overexpression,which promoted apoptosis.Conclusion Res attenuates he-patic inflammation and oxidative stress in rheumatoid arthritis via the Nrf2/Keap1 pathway.关键词
类风湿关节炎/肝损伤/白藜芦醇/炎症/氧化应激/Nrf2/Keap1Key words
rheumatoid arthritis/liver injury/resvera-trol/inflammation/oxidative stress/Nrf2/Keap1引用本文复制引用
范学菲,陈晓宇,周剑,陈苏环,张梦妍,刘浩淼,苏蕊,陈广祎,邵玉宝,姚涛..白藜芦醇通过Nrf2/Keap1途径减轻类风湿关节炎肝脏炎症和氧化应激[J].中国药理学通报,2025,41(5):861-867,7.基金项目
国家自然科学基金资助项目(No 81373421) （No 81373421）
安徽医科大学第三附属医院基础与临床合作研究提升计划培育专项(No 2022sfy010) （No 2022sfy010）
安徽医科大学"早期接触科研"项目(No 2024-ZQKY-078,2023-ZQKY-093) （No 2024-ZQKY-078,2023-ZQKY-093）
安徽省自然科学基金项目(No 2208085MH212) （No 2208085MH212）
