中国药理学通报2025，Vol.41Issue(5)：867-873,7.DOI:10.12360/CPB202409062
基于TLR4/MyD88/NF-κB信号通路探讨豆甾醇抗肝细胞癌的作用机制
Study on mechanism of stigmasterol inhibiting hepatocellular carcinoma based on TLR4/MyD88/NF-κB signaling pathway
摘要
Abstract
Aim To utilize molecular docking and in vitro experiments to investigate the regulatory mecha-nism of stigmasterol on the TLR4/MyD88/NF-κB sig-naling pathway in hepatocellular carcinoma.Methods Molecular docking was performed using AutoDock to study the binding affinity of stigmasterol with TLR4,MyD88,and NF-κB.The effects on cell proliferation and migration were assessed through CCK-8,wound healing assays,and Transwell experiments,while colony formation ability was measured using a colony formation assay.Flow cytometry was employed to examine apop-tosis and cell cycle.Western blot analysis was used to evaluate the expression levels of TLR4,MyD88,and NF-κB proteins after stigmasterol treatment.Results Molecular docking indicated that stigmasterol exhibited favorable binding conformations with TLR4,MyD88,and NF-κB.In vitro,stigmasterol demonstrated inhibi-tory effects on proliferation,migration,invasion,and colony formation in hepatocellular carcinoma cells.Ad-ditionally,it promoted apoptosis,inhibited cell cycle progression,and reduced protein expression of the TLR4/MyD88/NF-κB signaling pathway(P＜0.05).Conclusion Stigmasterol suppresses the activity of hepatocellular carcinoma cells by modulating the TLR4/MyD88/NF-κB signaling pathway.关键词
豆甾醇/Toll样受体4/髓样分化因子88/核因子-κB/肝细胞癌/凋亡Key words
stigmasterol/Toll-like receptor 4/myeloid differentiation primary response gene 88/nuclear fac-tor-KB/hepatocellular carcinoma/apoptosis分类
中医学引用本文复制引用
于春,马燕花..基于TLR4/MyD88/NF-κB信号通路探讨豆甾醇抗肝细胞癌的作用机制[J].中国药理学通报,2025,41(5):867-873,7.基金项目
国家自然科学基金地区基金项目(No 81860821) （No 81860821）
甘肃省联合科研基金一般项目(No 23JRRA1523) （No 23JRRA1523）
