中国药房2025，Vol.36Issue(9)：1065-1070,6.DOI:10.6039/j.issn.1001-0408.2025.09.08
指纹图谱结合含量测定分析黄芪闷润和蒸润切制的量值传递规律
Analysis of the quantity transfer rules in processing of Astragalus membranaceus by moistening-soaking and steaming-soaking followed by cutting based on fingerprint combined with content determination
摘要
Abstract
OBJECTIVE To analyze the quantity transfer rule in the processing of Astragalus membranaceus before and after moistening-soaking and steaming-soaking followed by cutting.METHODS Three batches of A.membranaceus decoction pieces processed through moistening-soaking and steaming-soaking followed by cutting were prepared.The HPLC overlapping fingerprints of A.membranaceus and its decoction pieces were established through the Similarity Evaluation System of Chromatographic Fingerprints of TCM(2012 edition).Combined with the previous qualitative analysis results,the common peaks were identified,the changes of common peak area were analyzed,and the principal component analysis was carried out.The contents of calycosin-7-glucoside,astragaloside Ⅰ and astragaloside Ⅳ in A.membranaceus and its decoction pieces were determined by HPLC,and the content differences of each component in different samples were compared.RESULTS The results of fingerprint analysis showed that 17 common peaks were identified.After steaming-soaking and moistening-soaking of A.membranaceus,the proportion of common peak area in the decoction pieces changed compared with the original medicine(for example,in A.membranaceus steaming-soaking decoction pieces,the proportion of peak area of malonyl calycosin-7-glucoside and malonyl astragaloside Ⅰ decreased,while the proportion of peak area of calycosin-7-glucoside increased).The results of principal component analysis showed that A.membranaceus,and its decoction pieces after moistening-soaking and steaming-soaking followed by cutting were all clustered into one category respectively.The results of content determination showed that,compared with A.membranaceus,the average content of calycosin-7-glucoside in A.membranaceus moistening-soaking decoction pieces was significantly reduced(P＜0.05);the average contents of calycosin-7-glucoside and astragaloside Ⅳ in A.membranaceus steaming-soaking decoction pieces were significantly increased(P＜0.05);there was no significant difference in the average content of astragaloside Ⅳ in A.membranaceus moistening-soaking decoction pieces and astragaloside Ⅰ in the two decoction pieces(P＞0.05).CONCLUSIONS There are differences in the quantity transfer rules of A.membranaceus before and after moistening-soaking and steaming-soaking followed by cutting.Steaming-soaking followed by cutting may make the transformation of unstable components(such as malonyl calycosin-7-glucoside and malonyl astragaloside Ⅰ)more complete.关键词
黄芪/闷润切制/蒸润切制/指纹图谱/含量测定/量值传递规律/毛蕊异黄酮苷/黄芪皂苷Ⅰ/黄芪甲苷Key words
Astragalus membranaceus/moistening-soaking followed by cutting/steaming-soaking followed by cutting/fingerprint/content determination/quantity value transfer rule/calycosin-7-glucoside/astragaloside Ⅰ/astragaloside Ⅳ分类
药学引用本文复制引用
赵晨光,汤书婉,刘顺,吴旭彤,彭国平,李存玉..指纹图谱结合含量测定分析黄芪闷润和蒸润切制的量值传递规律[J].中国药房,2025,36(9):1065-1070,6.基金项目
江苏省自然科学基金面上项目(No.BK20211395) （No.BK20211395）
江苏省中医药科技发展计划面上项目(No.MS2022019) （No.MS2022019）
南京市医疗机构中药传统制剂立项项目(No.NJCC-ZJ-202343) （No.NJCC-ZJ-202343）
江苏省中医疫病研究中心开放课题(No.JSYB2024KF11) （No.JSYB2024KF11）
江苏省中医院院内课题(No.Y24061) （No.Y24061）
