中国药理学通报2025，Vol.41Issue(5)：888-898,11.DOI:10.12360/CPB202409006
circ_0071653靶向miR-197-3p调控食管鳞状细胞癌的增殖和转移
circ_0071653 targeted miR-197-3p regulates proliferation and metastasis of esophageal squamous cell carcinoma
摘要
Abstract
Aim To investigate the effects of circ_0071653 targeting miR-197-3p on the proliferation and metastasis of esophageal squamous cell carcinoma(ES-CC)cells.Methods The circular structure of circ_0071653 was confirmed by Sanger sequencing and ribo-nuclease R tolerance experiments.Real-time quantita-tive polymerase chain reaction(RT-qPCR)and tissue fluorescence in situ hybridization assay were performed to detect the circ_0071653 expression levels and ana-lyze its clinical relevance.Cell fluorescence in situ hy-bridization and nuclear cytoplasmic separation assays were used to verify the subcellular localization of circ_0071653 and miR-197-3p.Bioinformatics analysis,dual luciferase reporter gene and RT-qPCR assays were conducted to validate the interactions between circ_0071653 and miR-197-3p.Moreover,the cell counting Kit-8(CCK-8),colony formation,scratch,Transwell invasion and subcutaneous tumor formation in nude mice assays were used to evaluate the effects of circ_0071653 and miR-197-3p on cell viability,prolifera-tion,migration,and invasion and in vivo tumorigenesi-sability.Results Circ_0071653 was a circular RNA,which showed high expression in ESCC cell lines and tissues.The expression of circ_0071653 was signifi-cantly correlated with lymph node metastasis and clini-cal stage of ESCC patients.Circ_0071653 and miR-197-3p were mainly localized in the cytoplasm.The databases predict that circ_0071653 had complementa-ry binding sites with miR-197-3p,and their binding were confirmed by dual luciferase reporter geneand RT-qPCR assays.Moreover,the activity,proliferation,migration,invasion and in vivo tumorigenesis abilities of ESCC cells were significantly reduced after knocking down circ_0071653,and this effect could be reversed by downregulating the expression of miR-197-3p.Con-clusions Circ_0071653 promotes the malignant pro-gression of ESCC through targeted regulation of miR-197-3p.关键词
食管鳞状细胞癌/circ_0071653/miR-197-3p/增殖/迁移/侵袭Key words
esophageal squamous cell carcinoma/circ_0071653/miR-197-3p/proliferation/migration/in-vasion分类
基础医学引用本文复制引用
郑璇,戈艳蕾,甘俊清,金叶,崔逸爽,吴亚男,刘子情,孙国贵..circ_0071653靶向miR-197-3p调控食管鳞状细胞癌的增殖和转移[J].中国药理学通报,2025,41(5):888-898,11.基金项目
国家自然科学基金面上项目(No 82472636) （No 82472636）
河北省创新能力提升计划项目(No 235A2403D) （No 235A2403D）
华北理工大学公共卫生学院高水平科研创新团队建设计划(No KYTD202309) （No KYTD202309）
