中国药理学通报2025，Vol.41Issue(5)：926-935,10.DOI:10.12360/CPB202410088
长链非编码RNA AC087388.1靶向PABPC1对食管鳞癌作用机制
Mechanism of action of long non-coding RNA AC087388.1 targeting PABPC1 on esophageal squamous cell carcinoma
摘要
Abstract
Aim To explore the roles and mechanisms of long non-coding RNA(lncRNA)AC087388.1 and poly(A)binding protein cytoplasmic 1(PABPC1)in esophageal squamous cell carcinoma(ESCC).Meth-ods The expression level of AC087388.1 in ESCC tissues and cells was detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and fluorescence in situ hybridization experiments and its clinical relevance was analyzed.Cell counting kit-8(CCK-8),clone formation,scratch and Transwell inva-sion assays were used to detect the effects of knock-down of AC087388.1 on the cell viability,prolifera-tion,migratory,and invasion of ESCC cells respectively ability,and sub-localization in cells.RNA pull down and Western blot experiments were employed to verify the interaction between AC087388.1 and PABPC1 in ESCC cells.Salvage experiments were performed to detect the effect of AC087388.1 targeting PABPC1 on ESCC cells.Results AC087388.1 was highly ex-pressed in ESCC tissues and cells and positively corre-lated with clinical stage of ESCC patients,mainly local-ized in cytoplasm.Knockdown AC087388.1 inhibited ESCC cell viability,proliferation,migration and inva-sionability.PABPC1 was selected from the results of RNA Pull Down-MS experiments for subsequent experi-ments,and AC087388.1 was verified to bind to PAB-PC1 by RNA Pull Down and Western blot experiments.Overexpression of AC087388.1 was verified by rescue experiment to reverse the effects of knockdown of PAB-PC1 on ESCC cell viability,proliferation,migration and invasion.Conclusions High expression of AC087388.1 correlates with clinical stage and may be a risk factor for ESCC progression.AC087388.1 pro-motes the cell viability,proliferation,migration and in-vasive ability of ESCC cells by targeting PABPC1,which may be a novel biomarker for the diagnosis and treatment of ESCC.关键词
食管鳞状细胞癌/lnc-AC087388.1/细胞质多聚腺苷酸结合蛋白1/增殖/迁移/侵袭Key words
esophageal squamous cell carcinoma/lnc-AC087388.1/PABPC1/proliferation/migration/in-vasion分类
医药卫生引用本文复制引用
仲瀚,戈艳蕾,甘俊清,金叶,郑璇,刘子情,孙国贵..长链非编码RNA AC087388.1靶向PABPC1对食管鳞癌作用机制[J].中国药理学通报,2025,41(5):926-935,10.基金项目
国家自然科学基金面上项目(No 82472636) （No 82472636）
河北省创新能力提升计划项目(No 235A2403D) （No 235A2403D）
河北省教育厅河北实验教学及教学实验室建设项目(No 81) （No 81）
华北理工大学公共卫生学院高水平科研创新团队建设计划(No KYTD202309) （No KYTD202309）
