中国药理学通报2025，Vol.41Issue(5)：950-959,10.DOI:10.12360/CPB202407023
基于网络药理学与组分配伍理论探讨鳖甲-莪术有效组分协同抗三阴性乳腺癌的作用及机制验证
The synergistic effect and mechanism verification of effective components of Biejia-Ezhu against triple-negative breast cancer based on network pharmacology and component compatibility theory
摘要
Abstract
Aim To explore the compatibility and po-tential mechanism of effective components of Biejia-Ezhu against triple negative breast cancer(TNBC)and verify it by experiments.Methods Effective compo-nents and targets of Biejia-Ezhu were obtained by TC-MSP and Swiss Target Prediction.Disease targets of TNBC were obtained from OMMI and GeneCards data-bases.The PPI network was constructed using STRING database.GO and KEGG path enrichment analysis was performed using DAVID database.Cytoscape3.9.1 software was used to construct the"drug-component-target-disease"network,screen key targets and compo-nents for molecular docking,and further verify the com-patibility of key components and targets in vitro.Re-sults ① A total of 71 effective components were iden-tified in the Biejia-Ezhu drug pair.There were 146 drug targets associated with the disease.A total of 113 signaling pathways were identified by KEGG analysis.The 71 potential active components of Biejia-Ezhu mainly acted on key targets such as mTORC1,ULK1,TNF,EGFR,ESR1,STAT3,HIF1A,and PTGS2.Mo-lecular docking results showed that glycine and curcu-min were the key active components of Biejia-Ezhu,and both had strong docking activity against key target proteins mTORC1 and ULK1.②The results of in vitro experiment showed that glycine combined with curcu-min significantly inhibited the proliferation and clonal formation ability of TNBC cells(P＜0.05),up-regula-ted the expression of autophagy marker LC3 Ⅱ/Ⅰ,down-regulated the expression of EGFR,down-regula-ted the expression of pathway protein mTORC1,p-mTOR,p-ULK1,and promoted the expression of path-way protein ULK1(P＜0.05).Conclusion The key component of Biejia-Ezhu against triple-negative breast cancer is glycine-curcumin,the mechanism of which may be related to the regulation of the mTORC1/ULK1 signaling pathway to promote autophagy.关键词
鳖甲-莪术药对/三阴性乳腺癌/组分配伍/自噬/mTORC1/ULK1信号通路/作用机制Key words
Biejia-Ezhu/triple negative breast canc-er/component compatibility/autophagy/mTORC1/ULK1 signaling pathway/effect mechanism分类
医药卫生引用本文复制引用
冯豆豆,骆小珊,蒙雁云,赵晶哲,朱久龙,黄雅珍,谢青,凌湘力,谢甦..基于网络药理学与组分配伍理论探讨鳖甲-莪术有效组分协同抗三阴性乳腺癌的作用及机制验证[J].中国药理学通报,2025,41(5):950-959,10.基金项目
国家自然科学基金资助项目(No 2460883) （No 2460883）
凌湘力全国名中医传承工作室建设项目(国中医药办人教函[2022]245号) （国中医药办人教函[2022]245号）
贵州省科技计划项目(黔科合基础-ZK[2022]一般442),[2024]一般204) （黔科合基础-ZK[2022]一般442）
贵州省中医药管理局民族医药科学技术研究课题(No QZYY-2023-001) （No QZYY-2023-001）
