中国药理学通报2025，Vol.41Issue(5)：960-969,10.DOI:10.12360/CPB202409085
冠心宁注射液通过CCL21调节心脏免疫改善心肌梗死作用
Effect of Guanxinning injection on myocardial infarction by regulating cardiac immunity through CCL21
摘要
Abstract
Aim To investigate the mechanism of Guanxinning injection regulating cardiac immune mi-croenvironment to improve myocardial infarction in mice.Methods In this study,MI model was estab-lished by permanent ligation of left anterior descending coronary artery in mice.The mice were divided into five groups:sham operation group,model group,Guanxinning injection low dose group,Guanxinning in-jection high dose group and positive drug captopril group.Hearts were weighed,heart tissues were collect-ed,and Masson staining was used for pathological anal-ysis of heart tissues;immunofluorescence staining was used to detect apoptosis and CCL21 expression in the infarct border zone;flow cytometry was used to detect the proportion of immune cells in myocardial ischemia tissues and lymph nodes;PCR was used to detect CCL21 expression in heart and in vitro human lymphat-ic endothelial cells(HLEC).Results Compared with the model group,the low and high dose groups of Guanxinning injection significantly improved cardiac hypertrophy.Apoptosis in the border zone of myocardi-al infarction was reduced in the low and high dose groups of Guanxinning injection and captopril group.Compared with the model group,the proportion of leu-kocytes in the infarct border zone was dreduced and the proportion of CD4+T cells,Treg cells,and CD8+T cells in the mediastinal lymph nodes and infarct border zone of the heart was regulated in the low and high dose groups of Guanxinning injection;CCL21 secretion by the heart and lymphatic vessels increased.Conclu-sions Guanxinning injection can significantly improve cardiac hypertrophy and fibrosis in MI mice,reduce ap-optosis in the infarct border zone,and play a role in an-ti-myocardial ischemia injury by promoting CCL21 ex-pression in lymphatic vessels to regulate the proportion of mediastinal lymph nodes and cardiac T cells after myocardial infarction.关键词
冠心宁注射液/心肌梗死/免疫/趋化因子配体21/淋巴管/T细胞Key words
Guanxinning injection/myocardial infarc-tion/immunity/CCL21/lymphatic/T cells分类
医药卫生引用本文复制引用
白雨欣,张迎雪,石婷婷,朱思楠,许振坤,王虹,陈璐..冠心宁注射液通过CCL21调节心脏免疫改善心肌梗死作用[J].中国药理学通报,2025,41(5):960-969,10.基金项目
国家自然科学基金资助项目(No 82174205,82274135) （No 82174205,82274135）
天津市科技项目(No 23ZYJDSS00020,QN20230330) （No 23ZYJDSS00020,QN20230330）
