中国药房2025，Vol.36Issue(9)：1082-1086,5.DOI:10.6039/j.issn.1001-0408.2025.09.11
柠檬苦素对妊娠期糖尿病大鼠肾脏病变及糖代谢、炎症和氧化应激的影响及机制
Effects and mechanism of limonin on renal lesion,glucose metabolism,inflammation and oxidative stress in gestational diabetic rats
摘要
Abstract
OBJECTIVE To explore the effects of limonin on renal lesions,glucose metabolism,inflammation,and oxidative stress in gestational diabetic rats and its possible mechanisms.METHODS The model of gestational diabetic rats was established by intraperitoneal injection of streptozotocin.The diabetic rats were divided into the model group(intragastrical administration and tail vein injection of equal volume of normal saline),limonin low-,medium-,and high-dose groups(intragastrical administration of limonin,at doses of 12.5,25.0 and 50.0 mg/kg,and equal volume of normal saline into the tail vein),and combination group[intragastrical administration of limonin 50.0 mg/kg+tail vein injection of c-Jun N-terminal kinase(JNK)activator Anisomycin 2 mg/kg],with 12 rats in each group.In addition,12 pregnant rats were selected as the control group(intragastrical administration and tail vein injection of equal volume of normal saline).They were given relevant medicine,once a day,for 14 consecutive days.After the last administration,fasting blood glucose(FBG),the levels of fasting insulin(FINS),interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α)in the serum were detected;the levels of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),blood urea nitrogen(BUN),and creatinine(Cr)in the renal tissue were detected;the pathological changes of renal tissue were observed;the expressions of proteins related to the JNK/nuclear factor-κB(NF-κB)signaling pathway in the renal tissue were detected.RESULTS Compared with control group,in model group,the rats showed pathological injuries in the kidney tissue,such as glomerular atrophy,edema of renal tubular epithelial cells;the levels of FBG,FINS,IL-1β,IL-6,TNF-α,MDA,BUN and Cr,HOMA-IR,as well as the phosphorylation levels of JNK and NF-κB p65 proteins were increased significantly(P＜0.05),while the levels of SOD and GSH-Px were decreased significantly(P＜0.05).Compared with model group,in each dose group of limonin,the degree of renal tissue lesions in rats was alleviated,and the above-mentioned indicators were significantly improved(P＜0.05),showing an obvious dose-effect relationship(P＜0.05).Compared with high-dose limonin group,in the combination group,the degree of renal tissue lesions in rats was relatively aggravated,and the changes in the above-mentioned indicators were significantly reversed(P＜0.05).CONCLUSIONS Limonin has a certain improvement effect on renal lesions,glucose metabolism,inflammation,and oxidative stress in pregnant rats with gestational diabetes.Its mechanism may be related to the inhibition of the JNK/NF-κB signaling pathway.关键词
柠檬苦素/妊娠期糖尿病/糖代谢/炎症/氧化应激/肾脏病变/JNK/NF-κB信号通路Key words
limonin/gestational diabetes/glucose metabolism/inflammation/oxidative stress/renal lesions/JNK/NF-κB signaling pathway分类
医药卫生引用本文复制引用
曲娜,张凯,那丽莎,崔莹..柠檬苦素对妊娠期糖尿病大鼠肾脏病变及糖代谢、炎症和氧化应激的影响及机制[J].中国药房,2025,36(9):1082-1086,5.基金项目
黑龙江省中医药科研项目(No.ZHY2022-042) （No.ZHY2022-042）
