中国现代医学杂志2025，Vol.35Issue(9)：92-98,7.DOI:10.3969/j.issn.1005-8982.2025.09.014
脑梗死患者外周血线粒体DNA拷贝数与病情严重程度的相关性研究
Association between peripheral blood mitochondrial DNA copy number and disease severity in cerebral infarction patients
摘要
Abstract
Objective To investigate the association between peripheral blood mitochondrial DNA(mtDNA)copy number and disease severity in patients with cerebral infarction.Methods We enrolled 199 cerebral infarction patients diagnosed at Meizhou People's Hospital from January to November 2024.Patients were stratified by NIH Stroke Scale(NIHSS)scores into mild(≤4)and moderate-severe(>4)groups,and further divided into high and low mtDNA groups based on median copy number.Quantitative real-time PCR was used to measure mtDNA levels.Spearman correlation and multivariate logistic regression analyses were performed to assess relationships between mtDNA copy number and clinical parameters.Results Significant differences were observed in age,NIHSS scores,leukocyte and neutrophil counts between severity groups(all P<0.05).Moderate-severe patients showed significantly lower mtDNA copy numbers than mild cases(P<0.05).Patients with high mtDNA had lower NIHSS scores than those with low mtDNA(P<0.05).mtDNA copy number correlated negatively with NIHSS(rₛ=-0.446,P<0.001),leukocytes(rₛ=-0.204,P=0.004)and neutrophils(rₛ=-0.221,P=0.002),and positively with lymphocytes(rₛ=0.185,P=0.009)and triglycerides(rₛ=0.167,P=0.020).Multivariate analysis identified high mtDNA as an independent protective factor against severe infarction((OR)=0.496,95%CI:0.380,0.647,P<0.05).Conclusion Peripheral blood mtDNA copy number significantly correlates with cerebral infarction severity,suggesting its potential as a circulating biomarker for disease assessment.关键词
脑梗死/mtDNA拷贝数/NIHSS/严重程度/相关性Key words
cerebral infarction/mitochondrial DNA copy number/national institutes of health stroke scale/disease severity/correlation分类
临床医学引用本文复制引用
翁锐强,古晓东,赵俊丽,李霞,刘苏东..脑梗死患者外周血线粒体DNA拷贝数与病情严重程度的相关性研究[J].中国现代医学杂志,2025,35(9):92-98,7.基金项目
广东省自然科学基金(No:2024A1515010809) （No:2024A1515010809）
广东省医学科研基金(No:A2023154) （No:A2023154）
梅州市医药卫生科研课题资助项目(No:2024-B-081) （No:2024-B-081）
